大鼠增龄过程中下丘脑-垂体-性腺轴细胞内游离钙改变  

Changes of intracellular free calcium in hypothalamus-pituitary-genital axis of rats during aging

作　　者：白生华[1] 熊杰[2] 

机构地区：[1]成都医学院第一附属医院制剂中心,四川省成都市6105002 [2]成都军区总医院检验科,四川省成都市610083

出　　处：《中国临床康复》2005年第15期112-113,共2页Chinese Journal of Clinical Rehabilitation

基　　金：四川省教育厅自然科学青年基金项目(2000-B37)~~

摘　　要：目的:探讨下丘脑-垂体-性腺轴细胞内游离钙随年龄增长的变化规律。方法:实验于2001-07/12在解放军第三军医大学药理教研室完成。检测3,6,12,18,21月龄Wistar大鼠90只分成了3(青年),6(成年),12(中年),18(老年前期),21(老年)月龄组,每组16只。下丘脑、垂体、卵巢(雌性)、睾丸(雄性)细胞内游离钙水平,与上一月龄组指标作比较。结果:90只大鼠均进入结果分析。①随月龄增加,下丘脑和垂体细胞内游离钙逐渐增加。3月龄、6月龄、12月龄、18月龄和21月龄下丘脑细胞内游离钙水平分别为(137±13),(140±21),(197±18),(240±28)和(243±21)nmol/L;垂体细胞内游离钙水平分别为(110±19),(117±18),(159±21),(199±22)和(203±14)nmol/L;其中12月龄组与6月龄组、18月龄组与12月龄组差异有显著性(t=5.096～8.291,P<0.05～0.01)。②随月龄增加,卵巢和睾丸细胞内游离钙逐渐增加。3月龄、6月龄、12月龄、18月龄和21月龄卵巢细胞内游离钙水平分别为(101±17),(108±23),(145±19),(188±29)和(221±28)nmol/L;睾丸细胞内游离钙水平分别为(190±21),(192±22),(238±17),(289±26)和(311±20)nmol/L;其中12月龄组、18月龄组和21月龄组与上一月龄组比较,差异均有显著性(t=2.276～5.973,P<0.05～0.01)。结论:随月龄的增长。AIM:To investigate the age-related changing regularity of intracellular free calcium in hypothalamus-pituitary-genital axis. METHODS:The experiment was carried out in the Department of Pharmacology,Third Military Medical University of Chinese PLA from July to December 2001 Ninety 3,6,12,18 and 21-month-old Wistar rats were divided into 3-month(young),6-month(adult) 12-month (middle-aged),18-month(early aged) and 21-month (aged) brain groups with 16 rats in each group.The levels of intracellular free calcium in hypothalamus,pituitary,ovaries of female rats and testis of male rats were detected, and compared with those in the former month age group. RESULTS:All the 90 rats were involved in the analysis of results.①With the increase of month age,the levels of intracellular free calcium in hypothalamus and pituitary were gradually increased.The levels of intracellular free calcium in hypothalamus of rats in the 3,6,12,18 and 21-month-old groups were(137±13),(140±21),(197±18),(240±28),(243±21) nmol/L respectively,and those in pituitary were(110±19),(117±18),(159±21),(199±22),(203±14) nmol/L respectively;There were significant differences between the 12 and 6-month-old groups,also between the 18 and 12--month-old groups(t=5.096 to 8.291,P< 0.05 to 0.01).②With the increase of month age,the levels of intracellular free calcium in ovaries and testis were gradually increased.The levels of intracellular free calcium in ovaries of rats in the 3,6,12,18 and 21-month-old groups were(101±17),(108±23),(145±19),(188±29),(221±28) nmol/L respectively,and those in testis were (190±21),(192±22),(238±17),(289±26),(311±20) nmol/L respectively,and those in the 12,18 and 21-month-old groups were significantly different from those in the former month age groups(t=2.276 to 5.973,P< 0.05 to 0.01). CONCLUSION:With the increase of month age,the levels of intracellular free calcium in each link of hypothalamus-pituitary-genital axis are obviously increased,from 12 months of age,the level is significantly higher in

关 键 词：下丘脑-垂体-性腺轴 细胞内游离钙 大鼠 增龄过程 解放军第三军医大学 mol/L 12月龄 垂体一性腺轴 0.05 钙水平 6月龄 变化规律 年龄增长 老年前期 脑细胞内 细胞内钙 显著性 教研室 卵巢 睾丸 生殖腺 

分 类 号：R446[医药卫生—诊断学] R285.5[医药卫生—临床医学]