逆灸对随后佐剂性关节炎大鼠早期及继发期炎性细胞因子和局部足肿胀的影响  

Influence of preventive moxibustion on inflammatory cells and local feet swelling of rats with adjuvant arthritis at early and secondary stages

作　　者：李晓泓[1] 张露芬[1] 郭顺根[2] 解秸萍[1] 李学武[1] 李辉[3] 翟景慧[1] 李敬道[1] 朱文莲[1] 赵福建[2] 赵丽云[2] 

机构地区：[1]北京中医药大学针灸学院,北京市100029 [2]北京中医药大学基础医学院,北京市100029 [3]中日友好医院针灸科,北京市100029

出　　处：《中国临床康复》2005年第15期123-125,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金课题(90209026);教育部优秀青年骨干教师课题~~

摘　　要：目的:观察逆灸对随后佐剂性关节炎大鼠早期、继发期关节局部肿胀和炎症因子白细胞介素1β、肿瘤坏死因子α的影响。方法:实验于2004-08/12在北京中医药大学针灸学院针灸机理实验室进行。以随机数字表法将48只Wistar大鼠分6组:正常组、模型早期组、逆灸早期组、模型继发组、逆灸继发组和逆灸正常组各8只。正常组:不造模,不预灸;模型早期组:大鼠右后足掌皮下注射福氏完全佐剂0.1mL造成佐剂性关节炎模型,造模第3天取材;逆灸早期组:于造模前进行艾灸,大椎穴处施灸,每次1壮,隔日灸1次,共灸8次,并于灸结束后造模,造模第3天取材;模型继发组:造模,造模第16天取材;逆灸继发组:于造模前进行艾灸,并于灸结束后造模,造模第16天取材;逆灸正常组:正常大鼠进行艾灸,灸结束后取材。采用放射免疫、鼠足容积测量法,观察逆灸对随后佐剂性关节炎大鼠早期和继发期足肿胀率及血清的白细胞介素1β,肿瘤坏死因子α的影响。结果:48只大鼠均进入结果分析。①足肿胀率:模型组和逆灸组右足早期肿胀率均高于正常组(2.128±0.262)%,(1.301±0.250)%,(0.011±0.018)%,F=26.001,P<0.01,且逆灸组肿胀较正常组明显减轻(P<0.05);模型组和逆灸组右足继发期肿胀率均高于正常组(1.736±0.165)%,(1.156±0.230)%,(0.009±0.018)%,P<0.01,且逆灸?AIM: To observe the effects of preventive moxibustion on the local joint swelling and inflammatory factors of interleukin-1-beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) in rats with adjuvant arthritis (AA) at early and secondary stages. METHODS:The experiment was carried out in the laboratory of acupuncture mechanism,School of Acupuncture, Beijing University of Chinese Medicine from August to December 2004.Forty-eight Wistar rats were divided into 6 groups with the method of randomly number table: normal group, model early group, preventive moxibustion early group, model secondary group, preventive moxibustion secondary group and preventive moxibustion normal group with 8 rats in each group.Rats in the normal group were not made into models and received no preventive moxibustion; Rats in the model early group were made into AA models by using subcutaneous injection of Zhao's complete adjuvant (0.1 mL) at palm of right hindfoot, and the samples were prepared on the 3rd day; Rats in the preventive moxibustion early group were made into AA models after muxibustion at Tachui acupoint once every other day, one moxa-cone every time for 8 times,and the samples were prepared on the 3rd day;Rats in the model secondary group were made into AA models, and the samples were prepared on the 16th day; Rats in the preventive moxibustion secondary group were made into AA models after muxibustion, and the samples were prepared on the 16th day; Rats in the preventive moxibustion normal group were treated with moxibustion, and then the samples were prepared after that. Radioimmunoassay and cubage measurement of rats' feet were performed to observe the effects of preventive moxibustion on the feet swelling rate and serum IL-1βand TNF-αin AA rats at early and secondary stages. RESULTS: All the 48 rats were involved in the analysis of results.①The right feet swelling rate at early phase was higher in the model group and preventive moxibustion group than in the normal group [(2.128±0.262)%, (1.301±0.250)%, (0.011±0.01

关 键 词：关节炎大鼠 炎性细胞因子 佐剂性 逆灸 继发 早期 白细胞介素1β 肿瘤坏死因子α 足肿胀 Wistar大鼠 北京中医药大学 福氏完全佐剂 正常组 关节炎模型 肿胀率 炎症因子 局部肿胀 针灸机理 针灸学院 皮下注射 正常大鼠 放射免疫 

分 类 号：R285.5[医药卫生—中药学] R541.4[医药卫生—中医学]