黄芪和当归注射液对兔肾缺血再灌注损伤时腺苷三磷酸酶的影响(英文)  

Effects of astragalus and angelica injections on adenosine triphosphate-ase in renal injury induced by ischemia/reperfusion in rabbits

作　　者：李达兵[1] 赵春玲[1] 林海英[1] 李先华[1] 邬于川[2] 

机构地区：[1]泸州医学院生理教研室,肾功能保护研究室,四川省泸州市646000 [2]泸州医学院微生物与免疫学教研室,四川省泸州市646000

出　　处：《中国临床康复》2005年第15期222-224,共3页Chinese Journal of Clinical Rehabilitation

基　　金：四川省中医药管理局资助项目(9833)~~

摘　　要：背景:近年研究表明,黄芪和当归具有抗自由基的作用,对肾缺血再灌注损伤具有保护作用。目的:观察黄芪、当归注射液在肾缺血再灌注损伤中对腺苷三磷酸酶(ATP酶)的保护作用及机制。设计:以实验动物为研究对象的观察对比实验。单位:一所医学院的生理教研室及肾功能保护研究室。材料:本实验于2001-01/2001-03在泸州医学院生理实验室完成。选择健康成年日本大耳白兔33只,由泸州医学院实验动物中心提供,雌雄不拘,体质量(1.63±0.22)kg。按随机数字表法分为假手术对照组8只、单纯缺血再灌注组8只、黄芪注射液+缺血再灌注组(黄芪组)8只、当归注射液+缺血再灌注组(当归组)9只。方法:术前1d、手术当天、术后1d,黄芪组、当归组每天分别静脉注射药物(黄芪1.25g/kg、当归12.5g/kg),对照组和单纯缺血再灌注组每天注射生理盐水5mL/kg。肾缺血1h再灌注48h后,下腔静脉采血,取右肾上极组织置入30mL/L戊二醛固定,下极组织制成组织匀浆。电镜检查肾组织超微结构,测血清肌酐含量及肾组织中ATP酶活性。主要观察指标:肾组织超微结构、血清肌酐含量及肾组织中ATP酶活性。结果:单纯缺血再灌注组肾组织变性显著,黄芪组、当归组病变较单纯缺血再灌注组明显减轻。单纯缺血再灌注组血清肌酐含量明显高于假手术对照组(P<0.05);黄芪组、?BACKGROUND:It is indicated in researches of recent years that both astragalus and angelica act on anti-free radical and protect renal injury due to ischemia/reperfusion. OBJECTIVE:To observe the protection and its mechanism of astragalus and angelica injections on adenosine triphosphate-ase (ATPase) in renal injury due to ischemia/reperfusion. DESIGN:The observing controlled experiment based on experimental animals . SETTING:Physiological teaching &research room and teaching &research room of renal functional protection in a medical college. MATERIALS:The experiment was performed in Physiological Experimental Room of Luzhou Medical College from January 2001 to March 2001.Totally 33 Japanese big-ear white healthy adult rabbits of either sex were employed, provided by Experimental Animal Center of Luzhou Medical College,in the mass of(1.63±0.22) kg. According to random number table,they were divided in sham-operation control(8 rabbits),simple ischemia/reperfusion group(8 rabbits),astragalus injection+ischemia/reperfusion group (astragalus group)(8 rabbits) and angelica injection+ischemia/reperfusion group(angelica group)(9 rabbits). METHODS:One day before operation,on the day of operation and 1 day after operation,successively,intravenous medical injections (astragalus 1.25 g/kg,angelica 12.5 g/kg) were administrated in astragalus and angelica groups everyday respectively,and injection with physiological saline 5 mL/kg was applied in the control and simple ischemia/reperfusion group.In 48 hours reperfusion after 1 hour ischemia in kidney,blood sample was collected from inferior vena cava.The upper tissue of the right kidney was collected and fixed by placed in 30 mL/L glutaraldehyde and the lower tissue was prepared into homogenate.Ultrastructure of renal tissue was examined with electron microscope; serum creatinine level and ATPase activity in renal tissue were assayed. MAIN OUTCOME MEASURES:Ultrastructure of renal tissue,serum creatinine level and ATPase activity in renal tissue. RESULTS:In simple ischem

关 键 词：肾缺血再灌注损伤 腺苷三磷酸酶 当归注射液 Na^+-K^+-ATP酶 Mg^2+-ATP酶 CA^2+-ATP酶活性 组织超微结构 泸州医学院 肌酐含量 日本大耳白兔 实验动物中心 静脉注射药物 保护作用 肾功能保护 生理教研室 黄芪注射液 对照组 

分 类 号：R692[医药卫生—泌尿科学] R544.102[医药卫生—外科学]