通痹灵对于白细胞介素2及其受体α链影响的体内外实验(英文)  

Tongbiling for interleukin 2 and its receptorα-chain in vivo and in vitro

作　　者：陈光星[1] 刘清平[1] 黄清春[1] 叶柳忠[1] 陈纪藩[1] 曾耀英[2] 

机构地区：[1]广州中医药大学金匮教研室,广东省广州市510405 [2]暨南大学生命科学院组织移植与免疫中心,广东省广州市510632

出　　处：《中国临床康复》2005年第15期237-239,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金重点项目资助(39930230);广东省自然科学基金重点项目资助(020656);广东省中医药管理局课题资助(102019)~~

摘　　要：背景:通痹灵是由《金匮要略》中桂枝芍药知母汤加减而成,运用于临床治疗类风湿关节炎已十多年,拟从白细胞介素2及白细胞介素2受体α链(CD25)的角度,从体内和体外两个方面,阐明其调控细胞免疫以及抗类风湿关节炎的作用机制。目的:从体内体外两个方面,研究通痹灵对于胶原诱导性关节炎大鼠关节滑膜原代细胞分泌白细胞介素2,以及通痹灵总碱对于白细胞介素2受体的α链CD25表达的影响。设计:以实验动物为研究对象的完全随机设计,对照实验研究。单位:一所大学的金匮教研室,及另一所大学的生命科学院组织移植与免疫中心。材料:实验于2001-04/07在广州中医药大学第一附属医院中心实验室完成。清洁级Wistar大鼠36只,雄性,购自中山大学医学院实验动物中心,动物合格证号2000A050。体质量(110g±20)g。分为正常组,造模组,甲氨喋呤高剂量组,甲氨喋呤低剂量组,复方通痹灵高剂量组,复方通痹灵低剂量组,每组6只。方法:体内实验,采用胶原诱导性关节炎大鼠动物模型,容积法测量足肿胀度,原代滑膜细胞培养,放免法检测培养液上清,观察通痹灵对滑膜内白细胞介素2的影响;体外实验,分离大鼠淋巴细胞,佛波醇酯或刀豆蛋白体外刺激,双色免疫荧光标记,流式细胞仪检测,观察通痹灵总碱对CD3+,CD25+表达的影响。主要观察指标:复方通痹灵?BACKGROUND:Tongbiling is the modified guizhi shaoyao zhimu tang(Decoction of Cinnamon Twig,Peony and Anemarrhena) recorded in Synopsis of Prescriptions of the Golden Chamber.It has been used for rheumatoid arthritis(RA) for more than ten years. Here is the experimental study on effects of tongbiling on interleukin 2(IL-2) and its α-chain,IL-2Rα(CD25),to explain the mechanism of tongbiling in regulating the cellular immunity and resisting RA from the aspects of in vivo and in vitro. OBJECTIVE:To study the effect of tongbiling on IL-2 in collagen introduced arthritis of rats and the influence of total base of tongbiling on expression of IL2-Rα(CD25). DESIGN:A totally randomized and controlled experimental study based on the experimental animals SETTING:Department of Jinkui of a university and the center of organ transplantation and immunity of another university. MATERIALS:The experiment was carried out from April to July 2001 in the Central Laboratory of the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine.The 36 Wistar rats,clean degree,male,bought from the Animal Experimental Center of Medical College of Sun Yat-sen University,certificate No.2000A050,weighted(110±20) g,were divided into 6 groups with 6 in each:normal group,model group,methotrexate(MTX) high dosage group,MTX low dosage group,tongbiling high dosage group, and tongbiling small dosage group. METHODS:The experiment in vivo was done with the modeled rats of collagen-induced arthritis.The foot swelling was measured with volume method,the cells of primary synovium cultured,and the upper clear fluid of culture fluid tested with radioimmunoassay to observe the effect of tongbiling on synovial IL-2.The experiment in vitro was done in the way of separating the lymphocytes,giving extraneous stimulation with phorbol 12,13-dibutyrate(PDB) or concanavalin A(ConA),using bicolor immunofluorescence labeling, testing with flow cytometer to observe the influence of total base of tongbiling on the expression of CD3+,CD25+.

关 键 词：广州中医药大学第一附属医院 α链 白细胞介素2受体 体内外 关节炎大鼠 Wistar大鼠 胶原诱导性关节炎 双色免疫荧光标记 CD25^+ 通痹灵总碱 桂枝芍药知母汤 抗类风湿关节炎 流式细胞仪检测 CD3^+ 《金匮要略》 完全随机设计 

分 类 号：R197.4[医药卫生—卫生事业管理] R392.11[医药卫生—公共卫生与预防医学]