红花长寿花茎段及叶片的离体培养与快速繁殖  

Study on in vitro culture and micropropagation from the stem sections and t he leaves of Kalanchoe blossfeldiana with red flower

作　　者：林秀莲[1] 赖钟雄[1] 黄双龙[1] 吴金寿[1] 黄玉吉[1] 黄晓霞[1] 柯存祥[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《亚热带农业研究》2005年第2期1-5,共5页Subtropical Agriculture Research

基　　金：国家"948"计划资助项目(No.991029)

摘　　要：采用红花长寿花茎段和叶片作为外植体进行离体培养与快繁研究。结果表明,茎段和带叶柄的叶片可以直接诱导出芽;在MS+0.25mg·L-1NAA+1mg·L-1BA+2mg·L-1GA培养基上有利于腋芽诱导和丛生芽形成;在1/2MS+0.1mg·L-1NAA培养基中生根效果最好。Stem sections and leaves from the plants of Kalanchoe blossfeldiana with re d flower were used as explants for in vitro culture and micropropagation. Th e result showed that buds were directly induced from the stem section and the le aves with leafstalks; and the MS medium supplemented with 0.25 mg·L -1NAA, 1 mg·L -1BA and 2 mg·L -1GA was beneficial to the induction of axil lary buds and the formation of bud clusters; and the highest percentage of rooti ng was obtained on the 1/2 MS medium adding 0.25 mg·L -1NAA .

关 键 词：离体培养 长寿花 快速繁殖 叶片 茎段 红花 直接诱导 腋芽诱导 生根效果 培养基 外植体 芽形成 快繁 出芽 叶柄 

分 类 号：S68[农业科学—观赏园艺] Q943.1[农业科学—园艺学]