鸡T淋巴细胞IL－2的体外诱生及活性检测  

Induction of IL-2 from Chicken T Lymphocytes and ItsActivity Test

作　　者：李庆章[1] 刘忠贵[1] 

机构地区：[1]东北农业大学动物医学系

出　　处：《中国兽医学报》1994年第2期159-163,共5页Chinese Journal of Veterinary Science

摘　　要：经Ｌ１６（４５）正交试验选择，并经实验验证，鸡脾脏Ｔ淋巴细胞白细胞介素２（ＩＬ－２）体外诱生和活性检测的最优水平组合及适宜条件为２．５μｇ／ｍＬ伴刀豆蛋白Ａ（ＣｏｎＡ）、ＩＬ－２体外诱生时间２０ｈ、１×１０７／ｍＬ淋巴细胞、１０％小牛血清、靶细胞培养时间４８ｈ、４×１０６／ｍＬ靶细胞、靶细胞接触时间３６ｈ、５ｍｇ／ｍＬＭＴＴ、ＭＴＴ加入时间３ｈ和甲溶解时间２ｈ；胸腺Ｔ淋巴细胞ＩＬ－２体外诱生和活性检测的最优水平组合及适宜条件为５μｇ／ｍＬＣｏｎＡ、ＩＬ－２体外诱生时间４８ｈ，余同脾脏Ｔ淋巴细胞ＩＬ－２体外诱生及活性检测。比较试验表明，ＭＴＴ比色分析法和３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法有显著的直线回归关系。The IL-2 induction from chicken T lymphocytes and its activity test werestudied.It was shown that when the spleenic T lymphocytes were used, the best consti-tution and optimum eonditions were as follows :2.5 μg/mL Con A ,inductive time invitro 20 hours,4 ×106/mL target cells,interactive time of target cells with IL-2 36hours,5mg/mL MTT,additive time of MTT 3 hours,dissolving time of formazan particles 2 hours,In case of thymus T lymphocytes,based upon the selection of orthogonaltest and verification by experiment, the constitution and conditions were identical withthose in spleenic T lymphocytes but the Con A concentration was 5 mg/mL and induc-tive time 48 hours. The comparative test indicated that there was significant linear re-gression relation between the MTT colorimetric assay and the 3H-TdR incorporation.

关 键 词：鸡 T淋巴细胞 IL-2 MTT比色分析 

分 类 号：S831.1[农业科学—畜牧学]