多头绒泡菌(Physarum polycephalum)中期染色体结构的AFM成像研究  

Imaging of metaphase chromosome structures of Physarum polycephalum by atomic force microscopy

作　　者：孙迎春[1] 王宏达[2] 裴瑾[1] 李壮[2] 黄百渠[1] 

机构地区：[1]东北师范大学细胞与遗传研究所,吉林长春130024 [2]中国科学院应用化学研究所,吉林长春130022

出　　处：《分子科学学报》2005年第1期31-35,共5页Journal of Molecular Science

基　　金：教育部留学回国人员科研启动基金资助项目(2002);国家重点基础研究发展规划("973计划")项目(G1999053902);吉林省科技发展计划项目(200305433)

摘　　要：　采用原子力显微镜术(AtomicForceMicroscopy,AFM)首次观察到了近自然状态下多头绒泡菌(Physarumpolycephalum)中期染色体的整体结构和表面细微结构.染色体整体结构成像显示其边缘轮廓清晰圆滑,具有明显的着丝点;染色体表面高分辨率成像发现,中期染色体是由约230nm的染色质纤维经螺旋且在底部回转180°形成,同时表面有3个层次有序折叠的染色质纤维同时存在,它们分别是:30～40nm,60～70nm,90～100nm.由高度图和侧向力成像图都可推测它们是螺旋结构.这些结果进一步证实了中期染色体包装的高度有序性,多层次结构是由螺旋方式形成,同时表明AFM是在近自然状态下研究生物大分子结构的强有力工具.The whole structures and the surface details of a native metaphase chromosome of Physarum polycephalum were firstly observed and imaged by atomic force microscopy.The imaging picture showed that the chromosome has clear and slick edge and apparent centromere.Furthermore imaging revealed that it is also observed from a chromosome was established by folding of a macro-helix fiber about 230 nm rotating 180° at its end.Three levels of chromatin fibers on the surface of metaphase chromosomes were observed,they are 30~40 nm,60~70 nm,90~110 nm,respectively.They were inferred helical structures from height imaging and friction imaging.The results confirm that the packages of mataphase chromosomes are highly order and the chromosome structures at near native state as well.

关 键 词：多头绒泡菌 染色体 AFM成像技术 原子力显微镜技术 染色质纤维 分辨率 真核生物 

分 类 号：Q939.1[生物学—微生物学] Q343.2