双低甘蓝型油菜杂交种亲本指纹图谱构建和杂交种纯度鉴定  

Fingerprints Construction of Hybrid Parents in B rassica napus and Its Utilization in Hybrid Purity Test

作　　者：刘平武[1] 周国岭[1] 杨光圣[1] 傅廷栋[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室

出　　处：《作物学报》2005年第5期640-646,共7页Acta Agronomica Sinica

基　　金：国家 8 63计划资助 (2 0 0 1AA2 41111)。

摘　　要：利用SSR分子标记构建了18份恢复系和2 1份不育系共39份甘蓝型油菜杂交种亲本的DNA指纹图谱,筛选出H990 1和H990 5两个杂交种亲本的共显性SSR标记Na10G0 8,并用其鉴定了H990 1、H990 5两个大田生产F1 杂种群体的纯度。对比SSR和田间调查的鉴定结果表明,SSR标记鉴定可以识别更多的非杂交种单株,如母本因微粉导致的自交、花粉污染、机械混杂等带来的非杂交种单株。本文还就SSR标记用于DNA指纹图谱构建和杂交种纯度鉴定的可行性和优点进行了讨论。Utilization of heterosis is an important wa y of increasing yield potential in crop breeding programs. Application of cytop lasmic male sterility (CMS), compared with other ways of utilizing heterosis in rapeseed, is the main way. However, with the effect of temperature and light , the male sterile lines of polima cytoplasmic male sterility (pol CMS) can pro duce small amount pollens, which will lead to self-pollination of the sterile lines in hybrid seed production. And the high ratio of sterile plants in F 1 p opulations will reduce the yield. In order to test purity of hybrid and protect the parents of hybrid, an effective and accurate system must be set up. Many molecular markers, such as RAPD, SCAR and AFLP have been widely used in constr ucting the fingerprints of crop varieties. However, the fingerprints analysis and hybrid purity test in Brassica napus has rarely been reported by using S SR. In the investigation reported in this paper, the fingerprints of 39 B. nap us hybrid parents including 18 restorers and 21 sterile lines were constructed with SSR. 106 SSR primer pairs were screened on 3 materials (Y04, Y12, Y29) with great genetic diversity, and 16 primer pairs were selected for further ana lysis. A total of 73 bands were amplified (Table 2), and the certain band patte rns were just the fingerprints for each B. napus hybrid parents, which cou ld be used in identifying the factuality of the parents and the purity of hybrid . According to the DNA fingerprints, primer Na10G08 was chosen to test purity o f F 1 hybrids H9901 (8086A×Lun31R) and H9905 (8110A×8759R), and 3 bands were produced by this primer. That was “010” in the female parents 8086A and 8110 A, “101” in the male parents Lun31R and 8759R, and “111” in the hybrids (F ig .1 and Fig.2). A total of 178 hybrid plants in H9901 and 173 hybrid plants in H9905 were investigated. PCR amplification result showed that 168 F 1 hybrid plants in H9901 and 152 F 1 hybrid plants in H9905 were true hybrids. The hybr id purity was

关 键 词：甘蓝型油菜 杂交种亲本 指纹图谱 杂交种纯度 SSR 

分 类 号：S565.4[农业科学—作物学]