再生水有机提取物雌激素效应研究  

Study on Estrogenic Effect of Organic Extracts of Reclaimed Water in Tianjin

作　　者：刘克明[1] 周蕾[1] 王春花[1] 管彤[1] 曹仲宏[2] 胡伟[2] 王玉秋[2] 

机构地区：[1]天津市卫生防病中心,天津300011 [2]南开大学环境科学与工程学院,天津300071

出　　处：《环境与健康杂志》2005年第3期174-176,共3页Journal of Environment and Health

基　　金：天津市自然科学基金资助项目(043606011);南开大学创新基金资助课题

摘　　要：目的研究天津市某再生水厂各处理单元水体的雌激素效应。方法采用反相C-18固相萃取柱提取各处理单元水样中目标物质,采用人乳腺癌细胞(MCF-7细胞)增殖试验和基因重组酵母试验检测水样有机提取物的雌激素效应。结果2项试验中各处理单元水样有机提取物均表现出雌激素效应,且在一定范围内,呈现出明显的剂量-效应关系。二级出水、混凝后出水、连续膜过滤后出水和O3处理后出水均在相当于原水1.00ml的浓度水平时表现出对MCF-7细胞最强的增殖效应,最大相对增殖效应依次为33.87%,19.49%,20.42%和16.24%。而且上述水样有机提取物均在相当于原水20ml的浓度水平时具有最高β-半乳糖苷酶活力(8.15,7.50,6.07和5.33)。结论天津市某再生水厂现有处理单元对水体雌激素效应具有有限的去除作用,但最终出水仍具有一定的雌激素效应。Objective To study the estrogenic effect of organic extracts of effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Methods Reverse phase C-18 solid-phase extraction (RP-C18SPE) was used for the extraction of target compounds from water samples. Then, a simplified proliferation test with human estrogen receptor-positive MCF-7 breast cancer cell and recombined yeast assay were applied to assess the estrogenic effect in effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Results The amount of extracts of reclaimed water inducing the maximum effect in cell proliferation assay was equal to that from 1.00 ml primary water. In recombined yeast assay, the amount of extracts of reclaimed water inducing maximum β-galactosidase activity was equal to that from 20.00 ml primary water. Conclusion The effluent of reclaimed water treatment plant still shows estrogenic effect though slightly reduced.

关 键 词：水污染 细胞增殖试验 基因重组酵母试验 再生水 雌激素效应 

分 类 号：R994.6[医药卫生—毒理学]