甲醛对原代培养大鼠皮质神经元谷氨酸受体2和γ-氨基丁酸受体蛋白表达的影响  

Effects of Formaldehyde on Protein Expressions of GluR2 and GABAR in Postnatal Rat Cortex Neurons in Culture

作　　者：刘婷 白雪涛 

机构地区：[1]中国疾病预防与控制中心环境与健康相关产品研究所,北京100021

出　　处：《环境与健康杂志》2005年第3期187-189,共3页Journal of Environment and Health

基　　金：科技部公益基金重点资助项目(2001DIA10001)

摘　　要：目的观察甲醛对原代培养大鼠皮质神经元谷氨酸受体2(GluR2)和γ-氨基丁酸受体(GABAR)蛋白表达的影响,探讨甲醛对神经元的毒作用机制。方法对原代培养的Wistar新生大鼠皮质神经元进行剂量分别为1.0、2.0、4.0、8.0mg/L的甲醛暴露实验,每小时染毒1次,连续染毒4h后,用免疫组化方法测定GluR2和GABAR的蛋白表达。结果与对照组相比,各实验组神经元GluR2蛋白表达均显著减少(P<0.01),并呈明显的剂量-效应关系,r=-0.768(P<0.01);GABAR蛋白表达均显著增加(P<0.01),也呈现明显的剂量-效应关系,r=0.856(P<0.01)。结论甲醛暴露可降低大脑皮质神经元GluR2蛋白表达,同时使GABAR蛋白表达增强。结果提示过量甲醛暴露可导致神经元细胞内钙超载,Cl-内流增加,生理功能受损。Objective To know the mechanism of the adverse effect of formaldehyde on cortex neurons. Methods In the present study, incubation of postnatal Wistar rat cortex neurons in culture with formaldehyde at 1.0, 2.0, 4.0, 8.0 mg/L (medium) was carried out to have knowledge of the effect of formaldehyde on the expressions of GluR2 and GABAR. For keeping the given concentration of formaldehyde, formaldehyde was added to the medium every hour for 4 consecutive hours. Results Immunocytochemistry showed a significant down-regulation of GluR2 and up-regulation of GABAR after 4 consecutive hours of formaldehyde treatment compared with the control (P<0.01), the significant doge-response relationships between formaldehyde and protein expressions of the two receptors were showed also(r=-0.768 and 0.856 respectively). Conclusion The results demonstrate that formaldehyde exposure in the one hand can induce down-regulation of GluR2 expression which may cause intracellular Ca2+ overload and in the other hand it can increase the expression of GABAR, then a increased influx of Cl- into neurons may happen.

关 键 词：甲醛 神经元 谷氨酸受体2 Γ-氨基丁酸受体 

分 类 号：R994.6[医药卫生—毒理学]