用umuC测试水中遗传毒性效应的样品前处理方法  

Improvement of Concentration Method for Detecting Genotoxic Activities of Drinking Water Using umuC-Test

作　　者：顾炜旻[1] 胡建英[1] 王伟[1] 刘燕花[1] 何文杰[2] 韩宏大[2] 

机构地区：[1]北京大学环境学院,北京100871 [2]天津市自来水集团有限公司,天津300040

出　　处：《环境科学》2005年第3期122-125,共4页Environmental Science

基　　金：国家高技术研究发展计划 (863 )项目 (2 0 0 2AA60 114 0 6) ;国家自然科学基金资助项目 (4 0 0 2 110 1)

摘　　要：umuC实验是用于检测物质致癌、致突变性的一种有效方法.本文将化学物质暴露阶段的温度细化为37℃,获得最佳灵敏度.以北方某自来水厂的自来水为对象,研究前处理方法对遗传毒性诱导效应的影响,发现以Oasis HLB固相萃取柱为浓缩柱,丙酮为洗脱溶剂的样品前处理方法显示出最高的遗传毒性诱导效应.对上述自来水厂不同工艺过程出水的遗传毒性进行检测,结果表明氯消毒过程促进遗传毒性的诱导.umuC-test is an effective method to determine the genotoxic activity. In this study, the highest genotoxic activity was found exactly at 37℃ by modifying original condition of umuC test. Effects of concentration methods on the genotoxic activity was studied, and it was found that the HLB solid phase extraction is most effective to extract the genotoxic chemicals from a drinking water when using acetone as eluant. The fate of genotoxic activity in drinking water treatment process was also studied, and chlorination promoted the induction of genotoxic activity.

关 键 词：umnC实验 遗传毒性 前处理方法 饮用水 

分 类 号：X832[环境科学与工程—环境工程]