高效抗逆转录病毒治疗对中国HIV/AIDS患者淋巴细胞活化及第二受体表达的影响  

Coreceptor exprssion and activation of T lymphocytes induced by highly active anti-retroviral therapy

作　　者：张子宁[1] 尚红[1] 姜拥军[1] 王亚男[1] 韩晓旭[1] 张民[1] 刘静[1] 刁莹莹[1] 代娣[1] 耿文清[1] 

机构地区：[1]中国医科大学附属第一医院艾滋病研究室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2005年第4期343-346,共4页Chinese Journal of Microbiology and Immunology

基　　金："十五"国家科技攻关课题资助( 2001BA705B01)

摘　　要：目的　了解高效抗逆转录病毒治疗后中国HIV/ AIDS患者淋巴细胞活化及CCR5、CXCR4表达的变化,探讨HIV感染者对于抗病毒治疗的免疫应答。方法　10例HIV /AIDS患者给予高效抗逆转录病毒治疗(HAART),用流式细胞仪检测治疗前和治疗第3、6 个月T淋巴细胞活化(HLA DR、CD38表达)及第二受体CCR5、CXCR4表达情况,比较HIV/ AIDS患者治疗前后淋巴细胞活化、第二受体表达的变化。结果　治疗前,10例HIV /AIDS患者CD4+、CD8+ T淋巴细胞活化水平均明显高于健康对照,CD8+ T淋巴细胞表面CCR5的表达明显高于健康对照,CXCR4 的表达明显低于健康对照(P<0.05);HAART治疗后,患者淋巴细胞活化水平随治疗时间明显下降(P<0.05),CD8+ T淋巴细胞表面CCR5表达水平显著降低(P< 0. 01), CXCR4 的表达升高;治疗6 个月时, CD38 CD4、HLA DRCD38 CD4、CCR5 CD8、CXCR4 CD8表达水平恢复至健康人水平;HIV AIDS淋巴细胞活化水平及第二受体CCR5的表达降低与HAART治疗后CD4+T淋巴细胞数量的升高具有显著的相关性。结论HAART能够降低中国HIV /AIDS患者淋巴细胞活化水平,使第二受体表达水平趋于正常,促进免疫功能的恢复。Objective To investigate the effect of HAART (highly active anti-retroviral therapy) on the activation and coreceptor expression of T lymphocytes of HIV/AIDS patients. Methods Ten HIV/AIDS patients were treated with antiviral drugs. Flow-cytometry was used to detect the activation and coreceptor CCR5, CXCR4 expressions in whole blood samples taken from normal controls and HIV/AIDS patients before and after therapy. Results The level of activation of T lymphocytes of the ten HIV/AIDS patients was significantly higher than normal controls before treatment. After HAART, the level of activation of T lymphocytes and the CCR5 expression on CD8+ T lymphocytes in HIV/AIDS patients significantly decreased. The decrease of activation and CCR5 expression of T lymphocytes in HIV/AIDS patients was significantly correlated with the increase of absolute counts of CD4+ T lymphocytes after HAART. Conclusion HAART can normalize the level of activation and coreceptor CCR5 expression in HIV/AIDS patients and induce the immune reconstitution.

关 键 词：HIV/AIDS患者 高效抗逆转录病毒治疗 受体表达 中国 T淋巴细胞活化 CXCR4 CD8^+ 流式细胞仪检测 CCR5 CD4^+ HIV感染者 第二受体 CD38 细胞表面 抗病毒治疗 HAART 治疗后 免疫应答 表达情况 治疗前后 治疗时间 细胞数量 

分 类 号：R512.91[医药卫生—内科学] R392.12[医药卫生—临床医学]