应用单细胞多重PCR进行性别鉴定  

Multiplex polymerase chain reaction assay for sex determination from a single cell.

作　　者：易萍[1] 李力[1] 姚宏[1] 俞丽丽[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所妇产科,重庆400042

出　　处：《中国优生与遗传杂志》2005年第5期23-24,共2页Chinese Journal of Birth Health & Heredity

基　　金：国家科技部 973项目 (2 0 0 1CB5 10 3 0 5 )

摘　　要：目的　建立一种准确、快速、简便的在单细胞水平进行性别鉴定的技术。方法　应用多重PCR同时扩增单个活检细胞的Y染色体特异重复序列(DYZ1)及X染色体特异重复序列(DXZ1)。结果　4 0个淋巴细胞(XY)成功地扩增出DXZ1电泳带(316bp)和DYZ1电泳带(15 4bp) ,正确率为10 0 % ;4 0个淋巴细胞(XX)中有39个成功地扩增出一条DXZ1电泳带(316bp) ,正确率为98%。结论　采用DYZ1和DXZ1引物对单个细胞同时扩增进行性别鉴定是一种简单可靠的方法,可用于胚胎种植前诊断及无创性产前诊断。Objetive:To develop a accurate and rapid and simple method to determine sex from a single cell. Methods:We developed a multiplex polymerase chain reaction assay from a single lymphocyte to co-amplify X(DXZ1)-and Y(DYZ1)-specific repeat sequences,yields a 316 bp band in females and two bands of 154 and 316 bp in males.Results:All 40 of the XY cells were amplified the DXZ1 and DYZ1 bands successfully and diagnosed as male(100%),where as 39 of the 40 XX cells were amplified the DXZ1 band and diagnosed as female(97.5%).Conclusion:This method could be used in preimplantation genetic diagnosis and noninvasive prenatal diagnosis.

关 键 词：多重PCR 性别鉴定 无创性产前诊断 胚胎种植前诊断 重复序列 淋巴细胞 单细胞水平 X染色体 Y染色体 方法应用 单个细胞 正确率 电泳 特异 

分 类 号：R715[医药卫生—妇产科学]