血管内皮细胞生长因子在大鼠心脏移植急性排斥期的表达研究  

The expression of vascular endothelial growth factor in rats cardiac transplantation

作　　者：徐鹏[1] 张向辉[1] 夏家红[1] 蒋雄刚[1] 张凯伦[1] 

机构地区：[1]华中科技大学协和医院心外科华中科技大学同济医学院心血管病研究所,武汉430022

出　　处：《临床心血管病杂志》2005年第5期287-289,共3页Journal of Clinical Cardiology

摘　　要：目的:观察血管内皮细胞生长因子(VEGF)在大鼠心脏移植急性排斥期中的表达及与排斥反应的关系。方法:实验分为对照组和环孢霉素A(CSA)组,每组27只。采用颈部心脏异位移植术式建立移植模型,于心脏移植手术后分别静脉给予0.85%氯化钠溶液及CSA干预。常规监测排斥反应发生情况。每组7只用于观察移植物存活时间,余20只移植术后1,3,7,11d各切取5例移植心标本。样本采用逆转录聚合酶链反应(RTPCR)的方法检测移植心VEGF的DNA表达水平。结果:CSA组移植心存活时间[(21.1±2.9)d]长于对照组[(12.4±2.3)d],P<0.01;对照组各采样时段VEGF的DNA表达强度均强于CSA组,P<0.01。结论:VEGF高表达与移植心的炎性浸润以及急性排斥有密切关系。Objective: To observe the expression and effect of vascular endothelial growth factor (VEGF) in rats cardiac transplantation. Method:Allografts were treated with CSA (group CSA, n= 27) or normal saline (control group, n=27) post operation immediately. After operation, allograft hearts sample were gotten separately at 1, 3, 7, 11d. The local expressions of VEGF were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) . Result:Allografts survival time was significantly longer in group CSA ( 21.1±2.9) d com- pared with that in control group (12.4 ±2.3) d. The expression level of VEGF is lower in CSA group compared with control group. Conclusion: The high-level expression of VEGF has proinflammatory function in accelerating the rejection of allograft.

关 键 词：血管内皮细胞生长因子 心脏移植 大鼠 移植排斥 

分 类 号：R392.114[医药卫生—免疫学]