一种可逆键合电泳微芯片的制作及在蛋白质分离中的应用  

Manufacture of Reversible Electrophoresis Chip and Its Application to Protein Detection

作　　者：庄贵生[1] 刘菁[1] 贾春平[1] 金庆辉[1] 赵建龙[1] 王惠民[2] 

机构地区：[1]中国科学院上海微系统与信息技术研究所,上海200050 [2]南通大学附属医院,南通226001

出　　处：《化学学报》2005年第11期1003-1007,i002,共6页Acta Chimica Sinica

基　　金：国家863(No.2002AA2Z2021)资助项目

摘　　要：阐述了一种可逆键合电泳微芯片的制作方法,以及电泳微芯片在蛋白质分离、临床尿蛋白检测方面的应用.用标准光刻腐蚀技术在石英基片上腐蚀泳道,清洗腐蚀好的基片和盖片后,在真空条件下实现键合.此种方法键合制作的电泳微芯片可重复键合使用,制得的电泳微芯片成功地用于标准蛋白质分离以及临床尿蛋白分析.This paper described a low-cost and reliable process for reversible bonding fabrication of electrophoresis microchips on quartz glass substrates in ordinary laboratory without the requirement of clean room facilities, and the microchips have been used in model protein separation and clinical urinary protein detection. Patterns of microchannel were fabricated through standard photolithography and etching on the quartz glass substrate. After bonding surfaces were rigorously rinsed, bonding was realized in vacuum ovens. The chip could be separated and re-bonded for usage. The microchip in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The electrophoretic buffer was 75 mmol/L borate with 0.5 mol/L lactate and pH 10.5. The chips have been used successfully for separation of model proteins and clinical urinary proteins.

关 键 词：蛋白质 分离方法 电泳微芯片 可逆键合 尿蛋白 制备方法 

分 类 号：O629.73[理学—有机化学] Q51[理学—化学]