大豆异黄酮活性组分对H_2O_2诱导的PC12细胞损伤的影响  

Effects of active ingredient of soybean isoflavoneson on PC12 cell injuries induced by H_2O_2

作　　者：陈伟强[1] 赵宇红[2] 罗少洪[3] 杨红[3] 金桂芳[3] 潘伟[3] 

机构地区：[1]广东药学院病理生理教研室 [2]广东药学院药理教研室 [3]广东药学院生物化学教研室

出　　处：《中国临床药理学与治疗学》2005年第4期443-446,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广东省科技厅计划项目 (№ 2 0 0 4B3 0 10 10 0 1) ;广东省中医药局科研项目 (№ 10 3 0 79)

摘　　要：目的:探讨大豆异黄酮活性组分对H2 O2 诱导的PC12细胞损伤的保护作用。方法:以H2 O2 损伤PC12细胞为氧化应激损伤的模型,采用光学显微镜观察细胞形态,MTT法判断细胞的存活率,LDH法检测细胞的损伤程度。结果:不同浓度大豆异黄酮能明显改善H2 O2 导致的细胞甲瓒减少,大豆异黄酮处理组细胞存活率明显高于H2 O2 处理组(P <0 .0 1) ;而LDH漏出率则明显低于H2 O2 处理组(P <0 .0 5 )。结论:大豆异黄酮活性组分对H2 O2 诱导的PC12细胞损伤具有保护作用。AIM: To explore the effects of active ingredient of soybean isoflavones on PC12 cells injuries induced by H_2O_2. METHODS: Active ingredients of soybean were extracted and purified. PC12 cells cultured in vitro was pretreated by different dosage of the ingredients about 12 hours and then injured by H_2O_2 (10 mmol·L -1 ). Cell viability rate in all groups was tested by MTT assay. Activity of LDH of all groups was tested. RESULTS: The viability rates in all groups pretreated was significantly increased compared with only H_2O_2 treatment group (P< 0.05 ). The activity of LDH in all pretreatment group was significantly decreased (P< 0.05 ) compared with H_2O_2 treatment group. CONCLUSION: Active ingredient of soybean isoflavoneson can restrain cytotoxicity induced by H_2O_2 and protect cells from peroxydation.

关 键 词：H2O2 PC12 大豆异黄酮 细胞损伤 阿尔茨海默病 

分 类 号：R971[医药卫生—药品]