伯氏疟原虫RC株和N株感染小鼠IFN-γ mRNA的表达及其对致病的影响  

Pathogenetic significance of IFN-γ mRNA expression in mice infected with RC and N strain Plasmodium berghei

作　　者：陈克强[1] 朱淮民[1] 宋关鸿[1] 

机构地区：[1]第二军医大学基础医学部病原生物学教研室

出　　处：《第二军医大学学报》2005年第5期515-518,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(39880041).

摘　　要：目的探讨伯氏疟原虫RC株和N株感染小鼠IFNγmRNA的表达及其对疟疾致病的影响。方法应用RT PCR技术,比较伯氏疟原虫RC株和N株感染后ICR小鼠脑、肝、脾组织IFNγmRNA表达的差异,并探讨其与原虫血症、体温和体质量等病理学参数之间的关系。结果N株感染小鼠后第10天,平均原虫血症达到(64.00±7.51)%,平均体质量降至(97.60±4.59)%,平均体温降至(27.87±2.95)℃,感染小鼠开始死亡;第12天小鼠全部死亡。RC株感染小鼠后第24天,平均原虫率达(61.10±6.56)%;第26天平均体质量和平均体温降至最低点;随后原虫率逐渐下降,第30天体质量和体温开始回升;感染后第36天,绝大多数感染鼠外周血涂片不能检出疟原虫,平均体质量和平均体温恢复正常;感染RC株小鼠的病死率仅为25%。感染N株小鼠在感染前期,肝、脾组织IFNγmRNA的转录明显增强,但持续时间较短;感染RC株小鼠在感染期间,肝和脾脏一直有IFNγmRNA转录,直到原虫被清除,小鼠完全康复。整个实验期间,正常对照小鼠肝和脾脏均未检测到IFNγmRNA。结论由N株和RC株疟原虫感染诱导小鼠肝、脾IFNγmRNA的表达可能具有不同的机制;RC株疟原虫感染可诱导小鼠肝、脾持续表达IFNγmRNA,对减轻疟原虫感染所造成的损害和感染鼠的康复有重要意义。Objective:To explore the relationship between the expression of interferon(IFN)-γ mRNA in RC and N strain Plasmodium berghei infected mice and the severity of malaria. Methods: Expression level of IFN-γ mRNA in the brain, liver and spleen of mice infected with RC or N strains of Plasmodium berghei was measured using RT-PCR,and the relationship between the transcription of the IFN-γ mRNA and parameters of malaria pathology including body weight, temperature and parasitemia was analyzed. Results: On day 10 post-infection,the average infected RBC(iRBC) was (64.00±7.51)%, average body weight fell to (97.60±4.59)%, average temperature dropped to (27.87±2.95)℃,and N strain infected mice began to die. On day 12, all N strain infected mice died. In RC strain infected mice, average iRBC reached ( 61.10±6.56)% on day 24 post-infection, and on day 26 average body weight([92.60±4.59]%) and average temperature([29.90±0.58]℃) dropped to the bottom.On day 30, the body weight and temperature began to rise while the parasitemia number decreased gradually. The parasites disappeared from the peripheral blood in most RC strain infected mice, and the average body weight and temperature were (105.00±5.80)% and (36.70±0.24)℃ respectively on day 36 after infection. The mortality of RC strain infected mice was only 25%. There was a temporally, obviously enhanced transcription of the IFN-γ mRNA in the liver and spleen of the N strain infected mice in the early stage of infection. The IFN-γ mRNA remained in the liver and spleen of the RC strain infected mice before the malarial parasites were eliminated from the bloodstream and the mice completely recovered from the malaria.No IFN-γ mRNA expression was detected in the liver and spleen of the control mice.Conclusion: There may be different mechanisms of inducing IFN-γ mRNA expression in the spleen and liver of ICR mice infected with N strain or RC strain.Expression of the IFN-γ mRNA at a certain level in the liver and spleen induced by the RC strain is maintained durin

关 键 词：伯氏疟原虫 RC株 N株 疟疾 干扰素 

分 类 号：R531.3[医药卫生—内科学]