STZ糖尿病大鼠心肌细胞凋亡及葡萄糖、血管紧张素Ⅱ、瘦素对心肌细胞凋亡影响的观察  

Observations of Cardiomyocytes Apoptosis in STZ-induced Diabetic Rats and the Effects of Glucose,Angiotensin Ⅱand Leptin on the Apoptosis of Cardiomyocytes

作　　者：翟迎九[1] 董雪红[1] 周丽诺[1] 胡仁明[1] 

机构地区：[1]复旦大学附属华山医院内分泌科

出　　处：《复旦学报（医学版）》2005年第3期330-332,336,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金资助 (3 0 2 70 62 7)

摘　　要：目的　观察链脲佐菌素(STZ)糖尿病大鼠心肌细胞凋亡情况及葡萄糖、血管紧张素Ⅱ、瘦素对正常SD大鼠心肌细胞凋亡的影响。方法　主动脉插管逆行灌流分离培养STZ糖尿病大鼠和正常SD大鼠心肌细胞,通过Annexin V FITC +PI双染法,采用流式细胞术,检测细胞凋亡情况,并运用细胞免疫荧光染色法检测心肌细胞膜瘦素受体的表达。结果　(1)STZ糖尿病大鼠心肌细胞凋亡较对照组明显增高(P <0 .0 1) ;(2 )在体外培养条件下,血管紧张素Ⅱ、瘦素使心肌细胞凋亡明显增加,且与浓度相关,差异均有显著性(P <0 .0 1) ,在葡萄糖5 .6、2 5mmol/L的培养环境中,心肌细胞凋亡变化不明显(P >0 .0 5 ) ;(3)细胞免疫荧光染色检测瘦素受体表达,在荧光显微镜下可见到心肌细胞膜呈现绿色荧光。结论　STZ糖尿病大鼠的心肌细胞凋亡增高;在体外培养条件下,血管紧张素Ⅱ、瘦素可促进SD大鼠心肌细胞凋亡,且与浓度相关,增加培养环境中的葡萄糖浓度,对SD大鼠心肌细胞的凋亡无明显影响,而瘦素可能通过与心肌细胞膜上瘦素受体结合而发挥作用。Purpose To observe the cardiomyocytes apo pt osis in STZ-induced diabetic rats and the effects of glucose,angiotensin Ⅱ and leptin on the apoptosis of primary cultured normal SD rats cardiomyocytes. Methods By perfusing retrogradely via the aorta,the car diomyocytes were isolated and cultured,the apoptotic cells were stained with An nexin-V-FITC and then quantified by flow cytometer.Meanwhile the expression of leptin receptor (OB-R) in cardiomycyte was identified. Results (1) The number of apoptotic cardiomyocytes in S TZ-induced diabetic rats increased significantly(P<0.01).(2) Incubated with different dose of angiotensin Ⅱ and leptin,the number of apoptotic cells incre ased obviously and dose-dpendently (P<0.01),incubated with concentratio n of 5.6 mmol/L and 25 mmol/L glucose,the apoptotic cells did not vary markedly (P >0.05).(3) By fluorescence microscope,fluorescence was observed on the membran e of cardiomyocytes,indicated that there was the expression of OB-R. Conclusions The number of apoptotic cardiomyocytes in S TZ-induced diabetic rats increased significantly.In vitro,angiotensin Ⅱ an d leptin can promote the apoptosis of SD rat cardiomyocytes,and it is dose-depe ndently,while elevated glucose concentration has no influence on the cardiomyocy tes apoptosis,leptin may produced a marked effect on the cardiomyocytes through the way of binding to OB-R.

关 键 词：心肌细胞凋亡 血管紧张素Ⅱ 瘦素 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]