Possible Effect of 30K Proteins in Embryonic Development of Silkworm Bombyx mori  

作　　者：Bo-XiongZHONG Jian-KeLI Jian-RongLIN Jian-SheLIANG Song-KunSU Hai-ShengXU Hai-YanYAN Ping-BoZHANG HiroshiFUJII 

机构地区：[1]LaboratoryofMolecularBiologyofSilkwormandHoneybeeDepartmentofBioresourceScience,CollegeofAnimalScience,ZhejiangUniversity,Hangzhou310029,China [3]LaboratoryofMolecularBiologyofSilkwormandHoneybeeDepartmentofBioresourceScience,CollegeofAnimalScience,ZhejiangUniversity,Hangzhou310029,China [5]InstituteofApiculturalResearch,ChineseAcademyofAgriculturalScience,Beijing100093,China [6]DepartmentofSericulture,SouthChinaAgriculturalUniversity,Guangzhou510642,China [7]BiochemistryCo.Ltd.ofZhejiangUniversity,Hangzhou310029,China [8]FacultyofAgriculture,KyushuUniversity,Fukuoka812-81,Japan

出　　处：《Acta Biochimica et Biophysica Sinica》2005年第5期355-361,共7页生物化学与生物物理学报（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.30271004);the Natural Science Foundation of Guangdong Province(No.021022)

摘　　要：The silkworm Bombyx mori possesses a 30K protein family of 3×10~4 Da,the biological functions of which have not been fully identified.The relationship between the 30K protein family and the embryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated by two dimensional polyacrylamide gel electrophoresis(2D-PAGE)and Matrix assisted laser desorption ionization- time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5 of the 30K protein family,mainly existing in normal strain,are possibly related to embryonic development.The early consumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3 and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development, which may lead to lower hatchability of the temperature sensitive strain.The results suggest that reasonable metabolism of 30K proteins is a prerequisite for the embryo's normal development.The silkworm Bombyx mori possesses a 30K protein family of 3×10~4 Da,the biological functions of which have not been fully identified.The relationship between the 30K protein family and the embryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated by two dimensional polyacrylamide gel electrophoresis(2D-PAGE)and Matrix assisted laser desorption ionization- time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5 of the 30K protein family,mainly existing in normal strain,are possibly related to embryonic development.The early consumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3 and 6G1-30K-4 are likely caused by the destruction of physiological balance in normal embryonic development, which may lead to lower hatchability of the temperature sensitive strain.The results suggest that reasonable metabolism of 30K proteins is a prerequisite for the embryo's normal development.

关 键 词：silkworm Bombyx mori 30K protein embryonic development 2D-PAGE MALDI-TOF MS 

分 类 号：S881[农业科学—特种经济动物饲养] Q969.435.1[农业科学—畜牧兽医]