Human embryonic stem cell lines derived from the Chinese population  

作　　者：ZhenFuFANG FanJIN HuiGAI YingCHEN LiWU AiLianLIU BinCHEN HuiZhenSHENG 

机构地区：[1]InvitroFertilizationCenterofWomen＇sHospital,SchoolofMedicine,ZhejiangUniversity,Zhejiang310006,China [2]CenterforDevelopmentalBiology,XinhuaHospital,ShanghaiSecondMedicalUniversity,1665KongJiangRoad,Shanghai200092,China [3]CenterforDevelopmentalBiology,XinhuaHospital,ShanghaiSecondMedicalUniversity,1665KongJiangRoad,Shanghai200092,China／／LaboratoryofStemCellBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutes

出　　处：《Cell Research》2005年第5期394-400,共7页细胞研究（英文版）

基　　金：This work was supported by grants from National Ba-sic Research Program of China(973 Program)(No:001CB509903,001CB509904);Hi-Tech Research and Development Program of China(973 Program)(No:2001A A216121.2004AA205010);National Natural Science Foun-dation of China(No:30040003);Science and Technology Committee of Shanghai Municipality(No:99DJ 14002,00DJI 4033,0IDJ 14003.03DJ 14017);Chinese Academy of Science(No:KSCX-2-3-08);Shanghai Municipal Edu-cation Commission and Shanghai Second Medical University.

摘　　要：Six human embryonic stem cell lines were established from surplus blastocysts. The cell lines expressed alkaline phosphatase and molecules typical of primate embryonic stem cells, including Oct-4, Nanog, TDGF1, Sox2, EBAF, Thy-1, FGF4, Rex-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. Five of the six lines formed embryoid bodies that expressed markers of a variety of cell types; four of them formed teratomas with tissue types representative of all three embryonic germ layers. These human embryonic stem cells are capable of producing clones of undifferentiated morphology, and one of them was propagated to become a subline. Human embryonic stem cell lines from the Chinese population should facilitate stem cell research and may be valuable in studies of population genetics and ecology.

关 键 词：human embryonic stem cell pluoripotent cell line teratoma. 

分 类 号：Q987[生物学—遗传学] Q25[生物学—人类学]