农杆菌介导的杜氏盐藻Dscbr基因转化紫花苜蓿的初步研究  

Transformation of Alfalfa (Medicago sativa L.) by Agrobacterium-mediated Process with Dscbr Genefrom Dunaliella salina

作　　者：陈晨[1] 乔代蓉[1] 白林含[1] 马梵辛[1] 贺顺姬[1] 曹毅[1] 

机构地区：[1]四川大学生命科学学院,成都610065

出　　处：《四川大学学报（自然科学版）》2005年第3期567-570,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家高技术863项目(2001AA212161)

摘　　要：利用农杆菌介导法进行紫花苜蓿遗传转化研究.将从盐生杜氏藻(Dunaliellasalina)中克隆到的抗逆基因Dscbr(编码一种早期光诱导蛋白)导入紫花苜蓿栽培种"中苜一号",获得52个转化株系.经过卡那霉素抗性鉴定和PCR分子检测,获得6株阳性苗,PCR阳性率为11.54%.结果表明Dscbr基因已整合到苜蓿的基因组中.Alfalfa cultivar ZhongMuYiHao was transformed with Dscbr gene from Dunaliella Salina via Agrobacterium-mediated process. 52 regenerated alfalfa plants were obtained . Kanamycin resistance and PCR amplification analysis showed that 6 putative transgenic plants had the Dscbr gene, and the transformation ratio was 11.54 percent. The stress resistance of putative transgenic plants in laboratory and in field will be tested.

关 键 词：紫花苜蓿 Dscbr基因 转基因植株 根癌农杆菌 

分 类 号：Q81[生物学—生物工程]