3种渗透剂对转基因烟草根系枯草芽孢杆菌纤溶酶(BSFE)分泌的调节  

Regulation of three penetrants on secretion of Bacillus subtilis fibrinolytic enzyme from transgenic tobacco′s root system

作　　者：王瑞刚[1] 张艳春[2] 张子义[1] 吴自荣[2] 王水平[2] 

机构地区：[1]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018 [2]华东师范大学生命科学学院,上海200062

出　　处：《植物资源与环境学报》2005年第2期1-5,共5页Journal of Plant Resources and Environment

基　　金：上海市科学技术委员会重大项目(9854190003)

摘　　要：用0.05%～8.00%的甘露醇、山梨醇和聚乙二醇6000等3种渗透调节剂可提高转枯草芽孢杆菌纤溶酶(Bacillussubtilisfibrinolyticenzyme,BSFE)转基因烟草(NicotianatabacumL.)根系BSFE的分泌表达水平,其水培液BSFE活性在15d内基本呈抛物线型变化趋势。经3种渗透剂处理后转BSFE基因烟草水培液的BSFE活性峰值明显高于对照,且出现时间比对照相对延迟1-2d。甘露醇、山梨醇和聚乙二醇6000可作为该转基因烟草根系BSFE分泌表达的有效化学调节剂。The different concentrations (0.05%-8.00%) of penetrants such as mannitol, sorbitol and polyethylene glycol 6000 can increase the expression and secretion level of Bacillus subtilis fibrinolytic enzyme (BSFE) from root system of transgenic tobacco (Nicotiana tabacum L.). The BSFE activity in liquid culture medium took the parabolic change tendency within 15 d cultured. After treated by three penetrants, the peak values of BSFE activity was apparently higher than that of the control, and the peaks appeared 1-2 d later than that of the control. Mannitol, sorbitol and polyethylene glycol 6000 can be used as the effective chemical regulators for BSFE expression and secretion from the transgenic tobacco′s root system.

关 键 词：枯草芽孢杆菌 烟草根系 纤溶酶 渗透剂 聚乙二醇6000 分泌表达 渗透调节剂 转基因烟草 化学调节剂 变化趋势 抛物线型 时间比对 甘露醇 山梨醇 E基因 活性 水培 

分 类 号：S572.01[农业科学—烟草工业] Q936[农业科学—作物学]