酪氨酸酶在自体中厚移植皮片中的表达  

Expression of tyrosinase in human skin autografts

作　　者：薛春雨[1] 邢新[1] 李蠡[1] 李军辉[1] 蒋平[2] 

机构地区：[1]第二军医大学长海医院整形外科 [2]第二军医大学生物化学与分子生物学教研室,上海200433

出　　处：《医学研究生学报》2005年第5期388-390,F003,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30371473)

摘　　要：目的:检测酪氨酸酶在人体自体中厚移植皮片中的表达,并与自身正常皮肤作对照,初步认识酪氨酸酶在自体移植皮片色素过度沉着中的作用。方法:利用免疫组化法检测人体自体中厚移植皮片与原供区及受区周围自体正常对照皮肤中酪氨酸酶的蛋白表达,并行统计学分析。结果:酪氨酸酶的表达定位于表皮基底部黑素细胞的细胞质,在大部分自体中厚移植皮片中呈强阳性表达,表达率为65.2%,与自体正常对照皮肤比较,差异有显著性意义(P<0.01);酪氨酸酶在受区与原供区周围正常对照皮肤中的表达差异无显著性意义(P>0.05)。结论:α黑素细胞刺激激素(αMSH)在自体中厚移植皮片中较自体正常对照皮肤中的表达显著增高,在自体移植皮片色素过度沉着中起重要的调控作用。Objective: To detect the expression of tyrosinase in human medium thickness skin autofrafts and compare with the normal skin, and to elucide the role of tyrosinase in hyperpigmented process of skin autografts. Methods: Immunohistochemical technique was performed to detect the expression and distribution of tyrosinase in medium thickness skin autografts and normal skin respectively.Statistics was used for analysis. Results: The expression of tyrosinase in most medium thickness skin autografts was much stronger than that of the control normal skin. The positive ratio of tyrosinase expression was 65.2% in medium thickness skin autografts, 17.4% in normal skin of donor area and 21.7% in normal skin around recipient area. The expression of tyrosinase in medium thickness skin autografts was significant different compared with normal skin(P<0.01). The expression of tyrosinase in normal skin of donor area was no statistic remarkably differences compared with normal skin around recipient area. Conclusion: The results indicate that the expression of tyrosinase was markedly increased in medium thickness skin autografts than in cortrol samples and was correlated with the pigmentation of skin autografts. Overexpression of tyrosinase may play an important role in hyperpigmented process of skin autografts.

关 键 词：酪氨酸酶 自体中厚移植皮片 色素过度沉着 

分 类 号：R655.8[医药卫生—外科学]