高效液相色谱法同时测定血浆中异烟肼和乙酰异烟肼  

Simultaneous determination of isoniazid and acetylisoniazid in plasma with HPLC

作　　者：曹晓梅[1] 陈冰[1] 冷伟卫[1] 李金恒[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)临床药理科,江苏南京210002

出　　处：《医学研究生学报》2005年第5期397-398,401,共3页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30472055)

摘　　要：目的:建立同时测定血浆中异烟肼和乙酰异烟肼的高效液相色谱(HPLC)法。方法:血浆样品经10%高氯酸沉淀蛋白后,取上清液直接进样分析。选用LichrospherC18柱(250mm×4.6mm,7μm),流动相为乙腈∶20mmol/L磷酸缓冲液(含2mmol/L庚烷磺酸钠)(2∶98,v/v),检测波长266nm。结果:异烟肼和乙酰异烟肼的血药浓度线性范围分别为0.12～15.89mg/L(r=0.998)和0.13～17.08mg/L(r=0.997),提取回收率分别为78.1%～95.6%和83.4%～94.4%,日内和日间变异系数(RSD)均<10%。结论:本研究建立的方法简便、准确,能满足药代动力学研究中生物样品测定的要求。Objective: To establish an HPLC method for simultaneous determination of isoniazid and acetylisoniazid in human plasma. Methods: Plasma was deproteinated by 10% perchloric acid. Isoniazid and acetylisoniazid were separated on a Lichrospher C18 column (250 mm×4.6 mm,7 μm) with UV detection at 266 nm. The mobile phase consisted of 20 mmol/L phosphate salt buffer containing 2 mmol/L IPR-B8 and 2% acetonitrile. Results: The linear concentration range for isoniazid and acetylisoniazid were 0.12-15.89 mg/L and 0.13-17.08 mg/L respectively (r>0.99), extraction recovery was 78.1%-95.6% for isoniazid and 83.4%-94.4% for acetylisoniazid. Within-day and between-day RSD were less than 10%. Conclusion: The method we established is simple, rapid and accurate. It is suitable for pharmacokinetical studies of isoniazid and acetylisoniazid.

关 键 词：异烟肼 乙酰异烟肼 高效液相色谱法 

分 类 号：R965.2[医药卫生—药理学]