糖尿病性勃起功能障碍大鼠血管紧张素Ⅱ及其受体变化的研究  

Effects of AngiotensinⅡ on Male Rats with Diabetic Erectile Dysfunction

作　　者：李三祥[1] 戴玉田[1] 

机构地区：[1]南京大学医学院附属鼓楼医院泌尿外科,江苏南京210008

出　　处：《中华男科学杂志》2005年第5期346-349,共4页National Journal of Andrology

基　　金：江苏省"135"工程重点医学人才基金(RC2001002);江苏省社会发展项目课题资金(BS2001066)资助

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)及其受体在雄性糖尿病性勃起功能障碍(DED)发病中的作用。方法雄性SD大鼠40只,随机取30只大鼠用于制作糖尿病模型,饲养8周后,从造模成功的17只糖尿病大鼠中筛选出8只有勃起功能障碍的大鼠(ED组);剩余9只未产生ED的糖尿病大鼠为DM组;正常对照组10只。3组大鼠麻醉后下腔静脉取血,放免法测定血浆中AngⅡ水平;取阴茎组织,1/4段用于免疫组化观察AngⅡ受体分布量的变化,剩余部分匀浆,测定组织匀浆中AngⅡ水平。结果与正常对照组相比,DM组血浆和海绵体组织及DED组血浆中AngⅡ水平明显升高(P<0.05);DED组阴茎组织中AngⅡ水平显著升高(P<0.01);各组大鼠阴茎组织AngⅡ受体分布随AngⅡ增高而降低。结论AngⅡ在DED大鼠血浆及海绵体组织中显著升高,在DED的发病中可能具有重要作用,血管紧张素转化酶抑制剂(ACEI)或AngⅡ受体拮抗剂有望成为治疗DED的重要方法之一。Objective: To investigate the changes of angiotensinⅡ(AngⅡ) and its receptors in male rats with diabetic erectile dysfunction(DED) so as to study its effects. Methods: Out of 40 male SD rats, 30 were taken at random for diabetic models. After 8 weeks, the rats with erectile dysfunction were selected from these models. All the rats were divided into 3 groups: control (n=10), diabetes mellitus(DM, n=9) and DED (n=8). For each rat, the AngⅡ levels in the blood and homogenate prepared from part of the isolated penile tissues were determined respectively, and the AngⅡ receptors of the rest of the penile tissues were analyzed through immunohistochemical(IHC) assay. Results: Compared with the control group, AngⅡ levels in the blood and penile tissues in the DM group, and that in the blood of the DED group were very high and the differences were statistically significant(P<~0.05 ). AngⅡin the penile tissues of the DED increased sharply(P<~0.01 ). The receptors of AngⅡchanged contrariwise to the level of AngⅡ. Conclusion: AngⅡ may play an important role in the pathogenesis of DED, and the AngⅡ antagonist or inhibitor of the angiotensin-converting enzyme (ACEI) may become a therapeutic method for DED in the future.

关 键 词：勃起功能障碍 糖尿病 血管紧张素Ⅱ 大鼠 

分 类 号：R587.2[医药卫生—内分泌]