340株肠球菌耐药性分析及基因分型  

Study on the antibiotic resistance and genotypes 0f 340 of enterococcus strains

作　　者：刘淑梅[1] 许淑珍[1] 

机构地区：[1]首都医科大学附属北京友谊医院检验科,在职研究生100050

出　　处：《北京医学》2005年第6期324-327,共4页Beijing Medical Journal

基　　金：北京市自然科学基金项目(编号7012013)

摘　　要：目的调查340株肠球菌的耐药性及其基因分型,了解医院感染的流行菌株。方法从痰、尿、血液中分离出不重复粪肠球菌340株,根据NCCLS推荐的琼脂稀释法进行药敏试验,并进行统计分析;采用随机扩增多态性DNA(RAPD)技术构建指纹图谱进行基因分型。结果粪肠球菌和屎肠球菌对青霉素、氨苄青霉素、万古霉素、高浓度庆大霉素的耐药率分别为18%、90%;14%、87.5%;1.3%、2.5%;51%、57.5%。300株粪肠球菌和40株屎肠球菌分别分为64个基因型和9个基因型,具有相同指纹图谱的菌株有5个组11株菌。结论肠球菌对多种抗菌药物具有较强耐药性;RAPD技术可用于肠球菌医院感染的分型研究,监测有无流行株在医院内暴发流行。Objective To investigate the resistance and genotypes of enterococci in order to provide guidance for clinical treatment and prevention. Methods Antibiotic sensitivity tests were performed by NCCLS recommended agar dilution method. WHONET?蛳5 software was used to analyse the resistance of enterococci. The RAPD technology was used for constructing the fingerprint mapping. The relationship was confirmed by the hierachical cluster analysis. Results The resistance rates of E. faecalis and E. faecium to penicillin, ampicillin, vancomycin, high?蛳concentration gentamycin were 18%, 90%; 14%, 87.5%; 1.3%, 2.5%; 51%, 57.5%, respectively. 300 Enterococcus faecalis strains were divided into 64 types,and 40 enterococci faecium were divided into 9 types. Five groups isolates(11 strains) had the same fingerprint map. Conclusions The resistance rates of enterococci to antimicrobial agents are high. RAPD technology can be used for studying the genotypes of enterococci in nosocomial infection.

关 键 词：基因分型 耐药性 分析及 随机扩增多态性 高浓度庆大霉素 粪肠球菌 RAPD技术 医院感染 指纹图谱 屎肠球菌 琼脂稀释法 NCCLS 氨苄青霉素 流行菌株 药敏试验 统计分析 万古霉素 抗菌药物 分型研究 暴发流行 基因型 耐药率 

分 类 号：R446.5[医药卫生—诊断学]