17β-雌二醇对肾上腺素诱导的心肌细胞肥大及原癌基因c-fos表达的影响  

Effects of 17β-estradiol on Phenylephrine induced Hypertrophy and Expression of Proto oncogene c-fos in Cultured Neonatal Rat Cardiomyocytes

作　　者：杨晓宁[1] 田宗文[1] 黄焕斌[2] 朱艳霞[1] 

机构地区：[1]武汉大学医学院人体解剖与组织胚胎学系,武汉430071 [2]湖北孝感市中心医院神经外科,孝感432100

出　　处：《医学新知》2005年第2期28-29,32,F004,共4页New Medicine

摘　　要：目的利用体外培养的乳鼠心肌细胞,观察17β雌二醇(17βestradiol)对肾上腺素(phenylephrine)诱导的心肌细胞肥大及其原癌基因cfos蛋白表达的影响。方法以培养的乳鼠心肌细胞为模型并分组给药后,用计算机图像分析软件测量心肌细胞表面积,免疫细胞化学方法检测心肌细胞原癌基因cfos的蛋白表达。结果17β雌二醇明显抑制肾上腺素诱导的心肌细胞表面积增大,同时减弱肥大心肌细胞原癌基因cfos的蛋白表达。结论17β雌二醇可抑制肾上腺素诱导的心肌细胞肥大,其作用机制可能和抑制原癌基因cfos的蛋白表达有关。Objective To observe the effects of 17β estradiol on phenylephrine induced hypertrophy and expression of proto oncogene c fos in cultured neonatal rat cardiomyocytes.Methods Using cultured neonatal rat cardiomyocytes as a model and after treating with different agents, surface area of cardiomyocytes was measured by computer photograph analysis software; the proto oncogene c fos protein expression was assessed with immunocytochemistry method. Results 17β estradiol inhibited obviouslyphenylephrine induced increase of surface area of cardiomyocytes; 17β estradiol reduced the proto oncogene c fos protein expression of hypertrophic cardiomyocytes. Conclusion 17β estradiol can inhibit hypertrophy of cardiomyocytes induced by phenylephrine and its mechanisms might be associated with the suppression of c fos protein expression.

关 键 词：17Β-雌二醇 心肌细胞肥大 肾上腺素 fos表达 原癌基因c-fos 免疫细胞化学方法 fos蛋白表达 细胞表面积 细胞原癌基因 肥大心肌细胞 计算机图像 体外培养 分析软件 同时减弱 作用机制 抑制 乳鼠 

分 类 号：R96[医药卫生—药理学]