犬细小病毒在乳猫肾细胞中的增殖动态  

Kinetics of canine Parvovirus Ams 20 Multiplication in Primary Feline Kidney Cells

作　　者：渠川玫[1] 徐在海[1] 彭传贵[1] 吴建华 郭淑杰 

机构地区：[1]军事医学科学院实验动物中心

出　　处：《中国畜禽传染病》1994年第5期5-7,共3页

摘　　要：犬细小病毒可在乳猫肾细胞内增殖．感染细胞培养液中病毒血凝滴度呈增长趋势，用生物素－亲和素酶间接染色法观察细胞在感染后１４小时．胞核中即可出现特异性染色．至７２小时胞核染色明显，有的细胞在包浆中亦出现特异性染色，此时感染细胞培养液可出现血凝现象．当胞浆染色明显时．病毒血凝滴度也增高．至感染晚期，胞核几乎空染，围绕胞核有一深色胞浆区，此时培养液的血凝滴度明显升高，两种方法所得结果相互印证．Abstract Canine Parvoviurs Ams 20,isolated from an infected dog with acute diarrhea.was inoculated into primary feline K idney cells.It was found that the culture fluids agglutinated porcine erythrocytes at 4℃,and the agglutination titer increased gradually after infection,and was inhibited by cpv antiserum.In the immunoperoxidasc studies,specifically stainedsubstance was detected first in the nuclei 14h postinfoction and the intensity reached themaximum 72h postinfection and viral antigen was also observed in the cytoplasm.Thespecific color in cytoplasm grew darker while the ucleus became balers On the I lth daypostinfectionl the nucleus became almost pale,this phenomenon rein.3ined to be furtherinvestigated.

关 键 词：犬 细小病毒 乳猫 肾细胞 增殖 

分 类 号：S858.292[农业科学—临床兽医学]