哺乳动物腔前卵泡体外培养及研究进展  

Progress on Culture and Development of Preantral Follicles In Vitro in Mammal

作　　者：梁洋[1] 刘娣 孙兴参[3] 岳顺利[3] 周佳勃[3] 

机构地区：[1]东北农业大学动物科技学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院,黑龙江哈尔滨150030 [3]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《动物医学进展》2005年第6期32-35,共4页Progress In Veterinary Medicine

基　　金：黑龙江省攻关项目(GB01B104);黑龙江省教育厅骨干教师资助计划

摘　　要：哺乳动物卵巢内的原始卵泡库是雌性生殖资源的储存库,但大部分卵泡在腔前阶段已退化闭锁了,这无疑是对这一资源的巨大浪费。因此,越来越多的学者致力于腔前卵泡的开发和利用,建立了一系列的培养体系,尤其在小鼠上做的比较成功,已经得到了少量后代。文章主要就卵泡的发生发育过程,腔前卵泡的分离方法,培养基的选择,不同直径腔前卵泡培养体系的建立,影响腔前卵泡发育的因素及研究进展进行了阐述,为进一步完善腔前卵泡培养体系,揭示其发育机理提供参考。Priordial follicles of mammalian ovaries are the banks of female gametes, but most follicles become atretic during pre-antral follicles, which is absolutely enormous waste. More and more scholars devote themselves to development and application of pre-antal follicles and several follicle culture systems have been successfully pioneered, especially in mice. In the article, follicle developmental process, methods of follicle isolation, culture systems of different diameter follicles, selection of medium, influential factors and the progress in their researches are summarized. It provides theoretic reference for modifying culture system of prantral follicles and discovers development mechanism of follicles.

关 键 词：哺乳动物 腔前卵泡 卵泡培养 

分 类 号：S857.2[农业科学—临床兽医学]