晕船易感大鼠与不晕大鼠脑干蛋白质双向电泳图谱的鉴定与分析  

Two dimensional electrophoretogram of brain stem protein of seasickness susceptible and non-seasickness rats

作　　者：杜鹏[1] 张建鹏[2] 苏波[1] 任绪义[2] 郭俊生[1] 

机构地区：[1]第二军医大学海军医学系军队卫生学教研室,上海200433 [2]第二军医大学基础医学部生物化学与分子生物学教研室

出　　处：《第二军医大学学报》2005年第4期411-414,共4页Academic Journal of Second Military Medical University

基　　金：军事医学专项基金(2001J 4).

摘　　要：目的:利用蛋白组学技术,建立晕船易感及不晕大鼠脑干总蛋白的双向电泳图谱,分析并鉴定差异表达蛋白质。方法:据模拟晕船刺激后异嗜高岭土量的增减将大鼠分为晕船易感组和不晕组,应用固相pH梯度(immobilized pH gradient,IPG)双向电泳(two dimensional electrophoresis,2 DE)技术,分离2 组大鼠脑干总蛋白,分析并用肽指纹图谱(peptide massfingerprint,PMF)鉴定差异蛋白质。结果:鉴定5个晕船易感相关蛋白质,上调酪氨酸3 单加氧酶/色氨酸5 单加氧酶激活蛋白、铁效应元件结合蛋白1,下调硫氧还蛋白过氧化物酶Ⅰ、磷酸甘油酸变位酶B、线粒体电压依赖型阴离子通道1。结论:成功建立分辨率和重复性高的晕船易感和不晕组大鼠2 DE图谱,研究表明差异蛋白质与神经递质合成、氧化损伤、能量代谢、细胞凋亡及铁代谢有关。Objective:To establish a two-dimensional electrophoresis(2-DE) system for total brain stem protein of seasickness susceptible and non-seasickness rats by using proteom technologies and to identify differentially expressed proteins. Methods: According to kaolin intakes after seasickness stimulation,rats were divided into seasickness susceptible group and non-seasickness group. The total brain stem proteins of both groups were isolated by immobilized pH gradient based two-dimensional electrophoresis. The differentially expressed proteins were analyzed by image analysis software and identified by peptide mass fingerprint(PMF) and database searching. Results: PMF identified 5 seasickness susceptible proteins,of which highly expressed proteins were tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activatiopro and iron-responsive element binding protein 1,while lowly expressed proteins included peroxiredoxin Ⅰ, phosphoglycerate mutase isozyme B protein and mitochondrial voltage dependent anion channel 1. Conclusion: A brain stem protein 2-DE electrophoretogram with high resolution and reproducibility has been successfully established. The differentially expressed proteins may play important roles in neurotransmitter synthesis, oxidation injuries, energy metabolism, cell apoptosis and iron metabolism.

关 键 词：晕船 蛋白质组 双向电泳 肽指纹图谱 

分 类 号：R835.1[医药卫生—航空、航天与航海医学]