西农萨能奶山羊CSN1S2基因多态与产奶量、体尺指标的相关分析  

Association of Polymorphisms of CSN1S2 Gene with Average Milk Yield and Body Sizes Indexes in Xinong Saanen Dairy Goat

作　　者：蓝贤勇[1] 陈宏[1,2] 张润锋[1] 田炎炎 张永德[1] 房兴唐[2] 孙维斌[1] 雷初朝[1] 胡沈荣[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]徐州师范大学生命科学学院生物技术研究所

出　　处：《畜牧兽医学报》2005年第4期318-322,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：教育部留学人员启动基金(1999);陕西省自然科学基金(99SM06);杨凌生物技术育种工程技术中心基金(199926)

摘　　要：利用PCR2RFLP技术对69只西农萨能奶山羊的CSN1S2基因进行多态性分析。扩增产物为310bp的CSN1S2F基因片段,被Alw26I限制性内切酶消化后表现多态性。F等位基因为310bp,N等位基因为179和131bp,相应地,FF基因型为310bp,NF基因型为310,179和131bp,NN基因型为179和131bp。在该群体中,F等位基因频率为010870,N等位基因频率为019130,处于Hardy2Weinberg平衡状态。将西农萨能奶山羊CSN1S2F基因座不同基因型与平均产奶量进行相关分析,结果表明:CSN1S2的不同基因型对平均产奶量有显著影响,NN基因型个体产奶量最高,FF基因型个体产奶量最低,且FF基因型个体平均产奶量显著低于NN基因型个体(P<0105)。将西农萨能奶山羊CSN1S2F基因座不同基因型与体尺指标进行相关分析,结果表明:在初生重和体高指标上,NF基因型个体显著优于NN基因型个体(P<0105);在体斜长和管围指标上,NF基因型个体略优于NN基因型个体,但差异不显著(P>0105);在成年体重和胸围指标上,不同的基因型个体间无差异。PCR-RFLP technique was used to analyze the polymorphisms of CSN1S2~F loci in 69 Xinong Saanen dairy goats. 310 bp fragment of CSN1S2~F gene digested with Alw26I demonstrated polymorphisms, namely, allele F with only one 310 bp band and allele N with 179 bp, 131 bp bands. Correspondingly, it showed that genotype FF of individuals had 310 bp band, genotype NF of individuals had 310 bp, 179 bp and 131 bp bands, and genotype NN of individual had 179 bp and 131 bp bands. In this Xinong Saanen goat population, gene frequencies of allele F and N were 0.087 0 and 0.913 0, respectively, which was at Hardy-Weinberg equilibrium (P>0.05). Analyzing correlation between different genotypes of CSN1S2 gene and average milk yield, we could find that different genotypes had significantly effect on average milk yield. In average milk yield index, milk yield of individual with genotype NN was the highest, and genotype NN were prior to FF genotype (P<0.05). Association different genotypes of CSN1S2 gene with body size indexes indicated that individual with genotype NF was better than those of genotype NN in body weight and stature (P<0.05), and individuals with genotypes NF and NN had no significant difference in other indexes (P>0.05), such as body size, shank girth and body weight.

关 键 词：萨能奶山羊 相关分析 体尺指标 产奶量 基因多态 等位基因频率 RFLP技术 限制性内切酶 基因型 多态性分析 Hardy 基因片段 扩增产物 平衡状态 基因座 个体 PCR FF 平均 初生重 体斜长 消化 体高 胸围 体重 

分 类 号：S827.32[农业科学—畜牧学] S826.92[农业科学—畜牧兽医]