2种锌源对体外培养胸腺细胞Bcl-2、Bax、Caspase-3mRNA表达的影响  

Effects of Zinc Sulfate and Zinc Methionine on Bcl-2, Bax, Caspase-3 mRNA Expression of Thymocytes in vitro

作　　者：虞泽鹏[1] 乐国伟[1,2] 施用晖[1,2] 

机构地区：[1]教育部食品科学与安全重点实验室 [2]江南大学食品营养与安全研究所,无锡214036

出　　处：《畜牧兽医学报》2005年第4期328-332,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：科技部农业成果转化项目(2002730020773)

摘　　要：采用地噻咪松作为诱导剂,建立小鼠胸腺细胞体外培养的凋亡模型,培养基中分别补加1000μmol/L的硫酸锌或蛋氨酸锌,培养16h。测定细胞的凋亡率、DNA片段化以及Bcl22、Bax、Caspase23mRNA表达量。结果表明,地噻咪松显著提高体外培养腺细胞凋亡(P<0101),并上调Bcl22、Bax、Caspase233种基因的mRNA表达(P<0101)。硫酸锌和蛋氨酸锌对地噻咪松诱导的凋亡均有显著的抑制作用(P<0101),并对上述3种基因表达的上调有抑制作用(P<0101)。蛋氨酸锌处理的细胞凋亡率及Caspase23mRNA表达均高于硫酸锌处理,Caspase23mRNA表达量差异达到了显著水平(P<0105)。这表明,锌调控糖皮质激素诱导的细胞凋亡涉及到对基因转录水平的调节,2种锌源在一定程度上存在着差异。To investigate the effects of different zinc sources on Bcl-2, Bax, Caspase-3 mRNA expression, dexamethasone was used to make the apoptosis model of thymocytes. Zinc sulfate and zinc methionine was added to the medium at the level of 1 000 μmol/L, the percentage of apoptosis nuclei were determined by flow cytometric analysis, Bcl-2, Bax, Caspase-3 mRNA were measured by RT-PCR. The results showed that both ZnSO 4 and Zn-Met can modulate apoptosis induced by dexamethasone, they inhibited apoptosis (P<0.01) and decreased DNA ladder happening; no difference was shown of modulating apoptosis between the two kinds of zinc (P>0.05). Both kinds of zinc could inhibit the up-regulating of Bcl-2, Bax, Caspase-3 mRNA induced by dexamethasone (P<0.01). Cell availability and Caspase-3 mRNA expression of cells cultured with Zn-Met were higher than those cultured with ZnSO 4 (P<0.05). The percentage of apoptosis nuclei, Bcl-2, Bax mRNA expression were similar within two groups (P>0.05). We draw the conclusion that both kinds of zinc could modulate apoptosis of thymocytes induced by glucocorticoid; the mechanism may involve the regulating ability of zinc on genes expression, and the two forms of zinc may go different ways in the regulations.

关 键 词：Caspase-3 mRNA表达 Bcl-2 体外培养 胸腺细胞 Bax 锌源 蛋氨酸锌 DNA片段化 细胞凋亡 抑制作用 硫酸锌 基因表达 显著水平 转录水平 激素诱导 表达量 诱导剂 mol 培养基 松 

分 类 号：S852.4[农业科学—基础兽医学] Q255[农业科学—兽医学]