胸腺五肽的NMR研究  

An NMR Study of Thymopoietin

作　　者：刘力军[1] 仲伯华[1] 缪振春[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《波谱学杂志》2005年第2期201-207,共7页Chinese Journal of Magnetic Resonance

摘　　要：应用改进的DPFGSE1D-TOCSY和1D-NOESY核磁共振方法测定胸腺五肽的氨基酸残基,从重叠的1H谱中分离出各自旋系统内氢的亚谱信号;继而由DPFGSE1D-NOE技术测定肽链氨基酸残基的连接顺序.结合二维相关谱对胸腺五肽的碳、氢信号做了全归属.并进行了计算机分子模拟,结果与NOESY实验相符.Severe spectral overlap occurs in the NMR spectrum of thymopoietin because the composing amino acid residuals have similar chemical shifts. In this paper, the structure of thymopoietin was determined by modified DPFGSE 1D-TOCSY and 1D-NOESY NMR techniques, which was then confirmed by computer simulation. The results demonstrate that DPFGSE 1D-TOCSY and 1D-NOE are efficient tools for structural elucidation of thymopoietin.

关 键 词：胸腺五肽 NMR 计算机分子模拟 氨基酸残基 核磁共振方法 二维相关谱 NOESY 应用改进 自旋系统 连接顺序 技术测定 ^1H 全归属 信号 氢 碳 

分 类 号：O482.532[理学—固体物理] TQ464.7[理学—物理]