板蓝根中邻氨基苯甲酸的抗内毒素作用  

Anti-endotoxic effects of o-aminobenzoic acid from Radix Isatidis

作　　者：刘云海[1] 李敬[1] 谢委[1] 丁玺[1] 陈雪琴[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《中南药学》2005年第3期138-141,共4页Central South Pharmacy

基　　金：国家自然科学基金资助项目(39170877;39870872);卫生部科学研究基金资助项目(982110)

摘　　要：目的研究板蓝根中邻氨基苯甲酸(oaminobenzoicacid,OABA)的抗内毒素作用。方法将邻氨基苯甲酸配成0.5%水溶液,鲎试验法进行抗内毒素定量测定;内毒素致家兔发热实验检测邻氨基苯甲酸体内抗内毒素作用;脂多糖(LPS)致小鼠死亡试验测定邻氨基苯甲酸保护作用及邻氨基苯甲酸对脂多糖致小鼠过度释放肿瘤坏死因子α(TNFα)和一氧化氮(NO)的影响试验,研究邻氨基苯甲酸的抗内毒素作用。结果0.833mg·mL-1邻氨基苯甲酸可使4EU内毒素降解为0.668EU,破坏率为84.4%;0.5%邻氨基苯甲酸溶液可使内毒素引起的家兔体温升高显著降低,使同等剂量脂多糖引起的小鼠死亡率从70%降为20%;板蓝根中的邻氨基苯甲酸可抑制脂多糖致小鼠血清中TNFα和NO的过度释放,其抑制率呈剂量依赖性。结论从板蓝根中分离出的邻氨基苯甲酸有抗内毒素作用。OBJECTIVE To determine the anti-endotoxic effect of o-aminobenzoic acid (OABA) isolated from Radix isatidis (Banlangen, BLG) . METHODS OABA was extracted and isolated from BLG and diluted into 0.5% solution. The content of OABA-pretreated endotoxin (ET) was quantitatively determined by Limulus test. The ability of fever induction of ET pretreated with OABA was measured using endotoxin-induced fever test in rabbits. The LPS-induced death in mice pretreated with and without OABA was compared. Influence on the LPS-induced release of TNF-α and NO in macrophages from mice was studied. RESULTS After pretreatment with OABA , 84.4% of ET was destroyed; the ET-induced fever in rabbits relieved markedly; and the LPS-induced death rate in mice dropped from 70% to 20%; inhibitive function for the release of TNF-α and NO induced by LPS in mice, the percentage of inhibition was dependent on the dosage when the concentration was between 0.125% ～0.5%. CONCLUSIONS OABA isolated from BLG had anti-endotoxic effects.

关 键 词：板蓝根 邻氨基苯甲酸 内毒素 脂多糖 

分 类 号：R285.5[医药卫生—中药学]