高羊茅成熟胚离体培养及高频植株再生  

High frequency plant regeneration from mature embryos in vitro culture of tall fescue

作　　者：何勇[1] 田志宏[1] 郑用琏[2] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]华中农业大学生命科学技术学院,湖北武汉430070

出　　处：《草业科学》2005年第6期23-29,共7页Pratacultural Science

基　　金：湖北省新世纪高层次人才工程科研项目(鄂人[2003]31号);湖北省重点科技发展计划项目(992P0603);湖北省教育厅重大项目(99Z007);湖北省自然科学基金项目(2000J0118)资助

摘　　要：以高羊茅Festucaarundinacea成熟种子为外植体,采用均匀设计研究了不同生长调节剂、基因型和培养基对愈伤组织诱导的影响。结果表明:2,4D对高羊茅成熟种子萌芽及愈伤组织诱导的影响极大;基因型对愈伤组织诱导有一定影响;培养基对愈伤组织诱导无任何影响;愈伤组织在不添加任何生长调节剂的培养基上即可进行分化。高羊茅最佳愈伤组织诱导培养基组合为N6+18mg/L2,4D,外植体为“王朝”成熟种子的愈伤组织诱导率可达95.6%,且植株分化率达100%。The effect of different growth regulators, different genotypes and different media on seed sprout and callus induction were studied with the uniform design model, and the mature seeds of tall fescue ( Festuca arundinacea ) were used as explants The results indicated that 2,4 D had significant effect on seed derived callus induction of tall fescue, the genotype had some effect on callus induction, however, no effect of medium upon callus induction, and the seed derived callus could differentiate immediately on medium without growth regulator The optimum medium supplemented with growth regulators for callus induction of tall fescue was 6 N+18 mg/L 2,4 D, and the callus induction frequency for the explants of mature seeds of “Dynasty” of tall fescue was up to 95 6%, and the plant differentiation rate reached 100%.

关 键 词：高羊茅 成熟胚 离体培养 愈伤组织 植株再生 均匀设计 

分 类 号：S543.9[农业科学—作物学]