HPLC法测定牛黄解毒片中大黄素的含量与方法耐用性的考察  

Determination of Emodin in Niuhuang Jiedu Pian by HPLC and Studies on Method Ruggeddness

作　　者：承新[1] 陈玉英[1] 胡育筑[1] 杨清华[1] 

机构地区：[1]中国药科大学分析化学教研室,中国科学技术大学生物系

出　　处：《中国药科大学学报》1994年第6期335-338,共4页Journal of China Pharmaceutical University

摘　　要：用ＨＰＬＣ法分离测定牛黄解毒片中大黄素的含量。用正交设计法考察了甲醇浓度、流动相ｐＨ值、流速、柱温以及检测波长对方法耐用性的影响。色谱柱：Ｓｐｈｅｒｉｓｏｒｂ　Ｃ（１８）（２５０ｍｍ×４．６ｍｍ，５μｍ），流动相：甲醇－水（Ｈ３ＰＯ４调ｐＨ为３．５）７８：２２，检测波长ＵＶ２５４ｎｍ，流速１ｍｌ／ｍｉｎ，大黄素的回收率为９８．２％，相对标准偏差ＲＳＤ＜２％。The content of emedin in Niuhuang Jiedu Pian was determined by HPLC.A 10μl portion was injected into a column (250 mm ×4.6 mm) of Spherisorb C(18)(5μm), with CH3OH-H2O(pH 3. 5)78:22 asmobile phase(1.0 ml/min)and UV detection at 254 nm, at 2 0℃. The effect of methanol concentration, pH of mobile phase, flow rate, column temperature and detection on the method ruggeddness was evaluated .The variation of methonal concentration caused the main effect.

关 键 词：大黄素 牛黄解毒片 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学]