山药水溶性多糖的化学及体外抗氧化活性  

Studyon Chemistry and Antioxidation Activity of Water Soluble Polysaccharides of Rhizoma Dioscoreas Oppositae

作　　者：何书英[1] 詹彤[1] 王淑如[1] 

机构地区：[1]中国药科大学生物化学研究室,南京第二制药厂研究所

出　　处：《中国药科大学学报》1994年第6期369-372,共4页Journal of China Pharmaceutical University

摘　　要：山药经热水提取、去蛋白、乙醇沉淀，ＳｅｐｈａｄｅｘＧ－１００纯化得山药多糖（ＲＰ），分子量为８．１×１０４。气相色谱分析ＲＰ主要含葡萄糖及少量岩藻糖。经过碘酸氧化及Ｓｍｉｔｈ降解后气相色谱分析结果显示各组分的摩尔比为岩藻糖－丙三醇－赤藓醇是１：１．２４：６．４２，证明ＲＰ主要由带有分枝的１→４连接的吡喃糖苷骨架构成，同时含有少量１→３键型的岩藻糖。该多糖能降低维生素ＣＮＡＤＰＨ及Ｆｅ（２＋）－半胱氨酸诱发的微粒体过氧化脂质的含量，并对黄嘌呤－黄嘌呤氧化酶体系产生的超氧自由基（Ｏ（２－））及Ｆｅｎｔｏｎ反应体系产生的羟自由基（ＯＨ－）有清除作用。The polysaccharide(RP) was isolated from Rhizoma Dioscoreae Oppositae with hot water and further purfied with Sephedex G-100.The average molecular weight of RP was 81000.This polysaccharide was composed mainl y of glucose and a trace of fucose by Gas Chromatography. After periedate oxidation and Smith degradation, the degraded products of RP were found to be fucose,glycerol, erythritol in molar ratio of 1:1.24:6.47.It suggested that RP mostly consisted of 1→4 linked glucopyranosyl backbone with branching points and a slight of amount of 1→3 linked fucose in materials,and RP could decrease the Malondialdehyde formation in the brain, liver and kidney microsome in rats that induced by NADPH-Vit C system and Cysteine-Fe(2+) system respectively;RP could also eliminate the superoxid e radical produced by Xanthine /Xanthin e Oxidase reaction system and hydroxyl redical produced by Fenton reaction system.

关 键 词：山药 多糖 自由基 抗氧化剂 抗衰老 

分 类 号：R286.76[医药卫生—中药学] R285[医药卫生—中医学]