白花前胡甲素对豚鼠心肌细胞钙电流的影响(英文)  

Effect of dl-praeruptorin A on calcium current in ventricular cells of guinea pig

作　　者：李金鸣[1] 常天辉[1] 孙晓东[1] 郝丽英[1] 王玉萍[1] 于艳凤[1] 张克义[1] 

机构地区：[1]中国医科大学药理学教研室

出　　处：《中国药理学报》1994年第6期525-527,共3页Acta Pharmacologica Sinica

基　　金：Science Foundation of the Ministry of Public Health, China,№ 88-402257;Part of this work was presented at the International Conference of Heart Research;1992 May 16-17; Beijing,China.

摘　　要：利用膜片钳制技术全细胞记录观察了白花前胡根中成分甲素对豚鼠单一心室肌细胞I_(Ca)的作用。表明;1,10,100μmol·L^(-1)的甲素使I_(Ca)的峰值变小,具有剂量依赖关系。给药前后I_(Ca)的翻转电位均为+60mV,实验结果提示甲素对Ca^(2+)通道有阻滞作用。With patch clamp technic (whole cell recording), the effect of dl-praeruptorin A ( Pra ) , an ingredient of Peucedanum praeruptorum Dunn on calcium current (Ica) in the single ventricular cells of guinea pig was studied. Results showed that under Cs/Cs condition, when the holding potential was - 40 mV and in the presence of Pra (1, 10, 100 μmol·L-1) , Ica was decreased dose-dependently from 2. 02 ± 0. 24, 2. 00 ± 0.12, 2.12 ± 0.33 nA (control) to 1.60 ± 0.24, 1.32 ± 0.08, 1.16 ± 0.43 nA, respectively, and their inhibitory rates were 21 %, 33; 5 % , 45 % , respectively. The current-voltage relation curve showed that the reversal potential of Ica was ± 60 mV; the potential producing peak value of Ica, was about 0 mV. The results indicated that Pra had a Ca2+ channel blocking effect.

关 键 词：白花前胡甲素 心肌细胞 钙拮抗剂 膜片钳技术 

分 类 号：R965.2[医药卫生—药理学]