丹参酮ⅡA磺酸钠对大鼠心，脑微粒体Na~＋，K~＋－ATP酶的抑制作用  

Inhibitory effects of sodium tanshinone Ⅱ A sulphonate onmicrosomal Na+, K+-ATPase in rat heart and brain

作　　者：王幼林[1] 陈念航[1] 丁建花[1] 

机构地区：[1]南京医科大学药理教研室

出　　处：《中国药理学与毒理学杂志》1994年第1期19-23,共5页Chinese Journal of Pharmacology and Toxicology

摘　　要：丹参酮ⅡＡ磺酸钠（ＤＳ－２０１）体外可逆性抑制大鼠心，脑微粒体Ｎａ＋，Ｋ＋ＡＴＰ酶。并具有浓度依赖性，且对心微粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶的抑制作用较扯降低Ｎａ＋或ＡＴＰ浓度则增强或减弱其抑酶作用，动力学分析表明ＤＳ－２０１对脑微粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶的作用近似Ｎａ＋的竞争性拮抗剂，ＡＴＰ的反竞争性拮抗剂，有Ｋ＋及Ｍｇ２＋存在时则表现为非竞争性或混合型抑制，但对心肌微粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶的作用则略有差别。The effects of sodium tanshinoneⅡ A sulphonate (DS-201) on the microsomal Na+,K+-ATPase from hearts and brains of rats were investi-gated in vilro. DS-201 inhibited the enzyme quicklyand reversibly. The inhibition was in a concentration-dependent manner and stronger on heart enzyme thanon that of brain. The DS-201 inhibition was enhancedat low Na+, but weakened at low ATP. Kinetic ana-lyses indicated the competitive nature of theinteraction between Na+ and DS-201 on the enzymeof brain. The DS-201 inhibition was uncompetitive forATP, noncompetitive or mixed type in the presence ofK+ and Mg2+ for the brain enzyme. However, thereare some differences in the action of DS-201 on theheart microsomal Na+. K+-ATPase.

关 键 词：磺酸类 丹参酮 脑 微粒体 

分 类 号：R965.1[医药卫生—药理学]