用反相离子对高效液相色谱法对三磷酸腺苷二钠及其制剂含量测定方法的研究  

Quantitative determination of Adenosine triphosphate and its prepara- tions by reversed-phase ion-pair HPLC

作　　者：陈海燕[1] 肖帆[1] 翟蔷 周淑平[1] 

机构地区：[1]北京市药品检验所

出　　处：《中国药学杂志》1994年第2期108-111,共4页Chinese Pharmaceutical Journal

摘　　要：用反相离子对高效液相色谱法对三磷酸腺苷二钠原料（以下简称ＡＴＰ）及４种制剂进行定性定量。色谱条件：流动相为１０％乙腈－水（含１５ｍｍｏｌＩＰＲ－Ａ）；紫外检测波长２５４ｎｍ；色谱柱为国产ＹＷＧ－Ｃ＿（１８）。线性范围０．４～２．０μｇ，相关系数γ＝０．９９９８。测定结果，ＡＴＰ原料、注射液、片剂、胶囊及注射用粉针回收率依次为１００．８％，９９．１１％，１０１．７％，９７．６２％及１０１．０％。本法具有快速灵敏，准确实用等特点。Adenosine triphosphate(ATP)and its four preparations were determined qualitatiely and quantitatively by reversed-phase ion-pair HPLC. Analvtical conditions included mobilephase:acetonitrile-water(1:9)containing 15mmol IPR-A;detecter wavelength:254nm;column:YWG-C18,(4mm×250mm);linear range:0.4～2.0μg;relative coefficient r=0.9998.The recov-eries of ATP raw rnaterial,injection,tablet,capsule and powder for injection were 100.8%, 99. 11%,101.7%,97.62%and 101.0%respectively.

关 键 词：高效液相色谱 腺苷三磷酸 含量 

分 类 号：R927.2[医药卫生—药学]