半边旗5F对瘢痕成纤维细胞增殖和凋亡的影响  

Effect of 5F from Pteris semipinnata L. on proliferation and apoptosis of scar fibroblasts

作　　者：张培华[1] 罗少军[1] 汤少明[1] 肖佐环[1] 左强[1] 李瑾[1] 

机构地区：[1]广东医学院附属医院整形外科,广东湛江524001

出　　处：《广东医学》2005年第6期773-775,共3页Guangdong Medical Journal

基　　金：教育部科学技术研究重点项目(编号:205114);广东省自然科学基金资助项目(编号:036736);广东省中医药局科研课题资助项目(编号:103074);湛江市2003年科技招标资助项目

摘　　要：目的通过对天然药物半边旗5F对瘢痕成纤维细胞凋亡作用的研究,以期探寻新的治疗病理性瘢痕的有效药物。方法以四甲基偶氮唑(MTT)法、流式细胞术和原位末端标记技术检测半边旗5F对成纤维细胞增殖和凋亡的影响。结果半边旗5F对成纤维细胞的增殖具有显著的抑制作用,且呈现浓度和时间依赖性。采用流式细胞术和TUNEL法检测凋亡指数,均呈剂量依赖性增高(P<0.05)。结论半边旗5F对瘢痕成纤维细胞的增殖具有显著的抑制作用,并且能够诱导其发生凋亡,为寻找有效治疗增生性瘢痕的药物进行了新的尝试。Objective To study the effect of herbal medicine Pteris semipinnata L on proliferation and apoptosis of scar-derived fibroblasts and search for drugs for human pathological scar. Methods Fibroblasts were cultured as an experimental model and the effect of Pteris semipinnata L was examined. Cell proliferation was determined by MTT assay, and apoptosis of fibroblasts induced by Pteris semipinnata L was analyzed by flow cytometry and TUNEL. Results Pteris semipinnata L significantly inhibited the proliferation of fibroblasts, and elicited apoptosis of cultured fibroblasts(P<0.05). Conclusion Pteris semipinnata L can significantly inhibit proliferation and induce apoptosis of cultured fibroblasts, suggesting its potential role as a new drug for the treatment of pathological scar.

关 键 词：瘢痕成纤维细胞增殖 半边旗 5F 流式细胞术 四甲基偶氮唑 TUNEL法 抑制作用 病理性瘢痕 时间依赖性 剂量依赖性 增生性瘢痕 凋亡作用 天然药物 有效药物 技术检测 末端标记 凋亡指数 有效治疗 

分 类 号：R619.6[医药卫生—外科学] R284.1[医药卫生—临床医学]