雷公藤内酯酮对小鼠腹腔巨噬细胞分泌NO和TNF-α的影响  

Effect of Triptonide on Secretion of NO and TNF-α in Mouse Peritoneal Macrophages

作　　者：何为[1] 潘建青[1] 曾叶[1] 任燕[1] 王辉丽[1] 杨业金[1] 

机构地区：[1]华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2005年第2期153-155,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No 30070899)

摘　　要：目的观察雷公藤内酯酮(T7)对活化的小鼠腹腔巨噬细胞(MΦ)分泌一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)的影响.方法分离、纯化巨噬细胞,给予不同浓度T7进行培养,以Griess试剂检测培养上清液中的NO含量,以MTT法检测培养上清液中的TNF-α活性.结果 T7在浓度0.4～3.2 mg/L、时间4～24 h范围内对MΦ分泌的NO和TNF-α具有显著抑制作用(P<0.01),且呈剂量和时间依赖关系.结论 T7可以抑制MΦ的活性.Objective To observe the effect of triptonide(T_ 7) on the secretion of nitric oxid (NO) and TNF-α in activated murine peritoneal macrophages (MΦs).Methods After being separated and purified,the MΦs were cultured with various concentrations of T_ 7 . The TNF-α activity and NO level of cellular supernatants were determined by MTT colorimetric assay and Griess reagent respectively.Results The level of NO and the activity of TNF-α in MΦs could be significantly inhibited (P<0.01) by T_ 7 (0.4-3.2 mg/L) during 4-24 h in a time- and dosage-dependent manner.Conclusion T_ 7 can inhibit the activity of MΦs.

关 键 词：雷公藤内酯酮 巨噬细胞 一氧化氮 肿瘤坏死因子 

分 类 号：R285.5[医药卫生—中药学]