低抗凝活性肝素修饰SOD的性质及抗炎活性  

Properties and anti-inflammatory activities of superoxide dismutasemodified with low anticoagulant activity heparin

作　　者：王凤山[1] 李素霞[1] 张天民[1] 

机构地区：[1]山东医科大学生化制药教研室

出　　处：《中国药学杂志》1994年第7期398-401,共4页Chinese Pharmaceutical Journal

摘　　要：用溴化氰活化低抗凝活性肝素对超氧化物歧化酶进行共价修饰制得修饰酶。经测定结合于酶蛋白上的低抗凝肝素的氨基己糖、己糖醛酸、硫醚基及抗凝效价均降低；修饰后超氧化物歧化酶活性降低；Ｃｕ、Ｚｎ部分丢失。对不同的炎症模型的抗炎试验表明，修饰酶具有较强的抗炎活性。Superoxide dismutase(SOD) was modified with low anticoagulant activity heparin(LAAH)which had been activated by CNBr,and a LAAH modified enzyme,LAAH_(CN)SOD,wasobtained.The aminohexose,hexuronic acid,sulfate group contents and the anticoagulant potencyof LAAH in LAAH_(CN)-SOD were lower than those of native LAAH,and the enzymatic activity ofLAAH_(CN)-SOD was also lower than that of unmodified SOD. The Cu Zn in SOD were partly lostin the course of modification.The experiments of anti-inflammatory effects on different inflamma-tory models,such as cotton-ball-induced inflammatory granulation in rats and the capillary per-meabilityincrease of abdominal cavity of mice induced bv acetic acid , showed that LAAH_(CN)-SODhad higher anti-inflammatory activity than both LAAH and SOD.

关 键 词：超氧物歧化酶 肝素 化学修饰 

分 类 号：R977.6[医药卫生—药品]