IL-18对感染柯萨奇B3病毒乳鼠心肌细胞的保护作用  

Preventive Effect of IL-18 on Cultured Myocardial Cells Infected with Coxsackie B3 Virus in Newborn Rats

作　　者：李玲[1] 刘亚黎[1] 彭华[1] 林雯[1] 陈立敏[1] 蒙冰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院儿科,武汉430022

出　　处：《华中科技大学学报（医学版）》2005年第2期238-240,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究IL-18抗柯萨奇B3病毒(Coxsackie B3 virus,CVB3)的作用.方法将心肌细胞分为4组.感染组:用CVB3感染心肌细胞;干预组:心肌细胞感染病毒后加入IL-18(4～30 ng/ml);IL-18组:正常心肌细胞加入IL-18(8 ng/ml);对照组:正常心肌细胞.观察培养心肌细胞病变(CPE)及心肌酶谱乳酸脱氢酶(LDH)和磷酸肌酸激酶(CK)的变化.并进行IL-18最大无毒剂量实验.结果感染组感染病毒后第2、5天心肌细胞病变程度较其余3组明显,LDH和CK浓度明显升高,与其余3组比较差异有极显著性意义(P<0.01).IL-18浓度≥40 ng/ml对正常心肌细胞有毒性作用;在4～20 ng/ml范围内对感染心肌细胞具有保护作用.结论 IL-18可以抑制CVB3对心肌细胞的损伤,对CVB3感染的心肌细胞有保护作用.Objective To study the effects of IL-18 against Coxsackie B3 viruses (CVB3). Methods The myocardial cells were divided into 4 groups: Infection group: the myocardial cells were infected only with CVB3; Treatment group: IL-18 (4-30 ng/ml) was given after the myocardial cells were infected with CVB3; IL-18 group: IL-18 (8 ng/ml) was added into the cultured myocardial cells; Control group: normal myocardial cells. The changes in the cultured cell pathogenic effect (CPE),LDH and CK were observed. The maximum nontoxic dosage test of IL-18 was performed.Results In infection group,the CPE of myocardial cells was aggravated and the levels of LDH and CK were significantly increased on the second and 5th day after virus inoculation as compared with other three groups (P<0.01).≥40 ng/ml IL-18 showed toxicity to normal myocardial cells. During a range of 4-20 ng/ml,IL-18 had protective effects on the infected myocardial cells.Conclusion IL-18 could inhibit the damage of CVB3 to the myocardial cells and had protective effects against the CVB-3-infected myocardial cells.

关 键 词：心肌炎 柯萨奇病毒B3组 白细胞介素18 心肌酶谱 乳酸脱氢酶 磷酸肌酸激酶 

分 类 号：R542.21[医药卫生—心血管疾病]