赛葵黄脉病毒元谋分离物卫星DNA结构特征  

Molecular characterization of a satellite DNA associated with Malvastrum yellow vein virus Yuanmou isolate.

作　　者：张弦[1] 江彤[1] 李桂新[1] 周雪平[1] 

机构地区：[1]浙江大学生物技术研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2005年第3期245-249,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30471137);国家杰出青年基金资助项目(30125032).

摘　　要：从云南元谋表现黄脉症状的杂草赛葵上分离到一病毒分离物Y200.对其DNAA基因组部分序列测定分析表明,该序列与赛葵黄脉病毒Malvastrumyellowveinvirus(MYVV)相应序列的同源性为96.1%.利用DNAβ的特异性引物,从Y200中扩增到卫星DNA分子.序列分析表明,该DNAβ全长1348个核苷酸,全序列与MYVVDNAβ的同源性最高,为96.1%.DNAA部分序列和DNAβ全长序列分析表明,该病毒分离物为MYVV.A virus sample Y200 showing yellow vein symptom was collected from Malvastrum coromandelianum in Yuanmou of Yunnan Province. Partial DNA-A sequence of Y200 is closely related to that of Malvastrum yellow vein virus (MYVV) DNA-A with 96.1% sequence identity. DNAβ molecule was amplified from Y200 with primers specific for DNAβ, Y200 DNAβ contains 1348 nucleotides, and it has 96.1% nucleotide sequence identity with MYVV DNAβ。 Comparisons of the partial DNA-A and complete DNAβ sequences conform that Y200 is an isolate of MYVV.

关 键 词：赛葵黄脉病毒 赛葵 DNAΒ 

分 类 号：S436.45[农业科学—农业昆虫与害虫防治]