不同生长环境下水稻主要农艺性状的QTL分析  

QTL Analysis of Some Agronomic Traits in Rice Under Different Growing Environments

作　　者：韩龙植[1] 乔永利[1,2] 张三元[3] 曹桂兰[1] 叶昌荣[4] 徐福荣[4] 戴陆园[4] 芮钟斗 高熙宗 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081 [2]首尔大学校农业生命科学大学,韩国首尔151-921 [3]吉林省农业科学院水稻研究所,公主岭136100 [4]云南省农业科学院生物技术与种质资源研究所,昆明650205 [5]韩国农村振兴厅作物科学院

出　　处：《中国农业科学》2005年第6期1080-1087,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30070421);国家"十五"科技攻关计划资助项目(2004BA525B02);中韩合作资助项目(2002~2005)

摘　　要：以籼粳交密阳23/吉冷1号200个F2:3家系作为作图群体,在北京(正常生长环境)、昆明(自然低温胁迫)、公主岭(冷水胁迫)、韩国春川(正常生长环境和冷水胁迫)等5种不同生长环境下进行了水稻秆长、穗长、穗粒数和穗抽出度等主要农艺性状的异地鉴定,并利用SSR标记进行了上述农艺性状的QTL分析。检测结果表明,5种不同环境下检测到与上述农艺性状相关的QTL共44个,分布于除第9染色体外的11条染色体上。qCL1a、qCL1b、qCL5a、qCL6b、qPL1a、qPL3a、qPL6b、qPL6c、qPL7b、qSP8b、qSP1c、qSP11a、qSP12和qPE1至少在2种不同生长环境下均检测到,受环境的影响较小,为较稳定的QTL。其中,qCL1a、qCL1b、qPL1a、qSP1c和qPE1对表型变异的解释率分别为24.2%～55.2%、22.7%～39.9%、12.5%～27.7%、14.4%～33.5%和26.6%～28.7%,为主效基因,主要表现为超显性。这些主效QTL在低温环境下对水稻秆长、穗长、穗粒数和穗抽出度有增效作用,与水稻耐冷性有一定的相关性。The culm length, panicle length, spikelets per panicle and panicle exsertion were evaluated by using F2:3 population including 200 lines, derived from a cross of indica and japonica Milyang23/Jileng1 under five different environments of Beijing (natural normal growing environment), Kunming (natural low temperature condition), Gongzhuling of China (cold water irrigation) and Chuncheon of Korea (natural normal growing environment and cold water irrigation), and QTLs of these traits were analysed by using SSR markers. The results showed that 44 QTLs related to these agronomic traits were detected under five different growing environments, and these QTLs are located on eleven charomosomes except chromosome 9. The QTLs for qCL1a, qCL1b, qCL5a, qCL6b, qPL1a, qPL3a, qPL6b, qPL6c, qPL7b, qSP8b, qSP1c, qSP11a, qSP12 and qPE1 have been detected in more than two growing environments, which were little affected by environments, were stable QTLs. Among them, qCL1a, qCL1b, qPL1a, qSP1c and qPE1 explained 24.2%-55.2%, 22.7%-39.9%, 12.5%-27.7%, 14.4%-33.5% and 26.6%-28.7% of observed phynotypic variation, respectively, which were major genes mainly appeared over dominance. These QTLs cause the increase of culm length, panicle length, spikelets per panicle and panicle exsertion under low temperature, showing that these QTLs are correlated with cold tolerance.

关 键 词：生长环境 QTL分析 农艺性状 水稻 SSR标记 作图群体 低温胁迫 检测结果 性状相关 表型变异 主效基因 低温环境 增效作用 水胁迫 穗粒数 籼粳交 染色体 相关性 耐冷性 稻秆 穗长 出度 F2 家系 显性 

分 类 号：S511[农业科学—作物学]