血红素氧合酶-1抑制T淋巴细胞对血管内皮细胞的粘附作用  

HO-1 protects endothelial cells primed interferon-γ from Jurkat cells adhesions

作　　者：杜敦峰[1] 昌盛[1] 陈必成[1] 周鸿敏[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究院,武汉430030

出　　处：《中华器官移植杂志》2005年第6期363-365,共3页Chinese Journal of Organ Transplantation

基　　金：973基金资助项目(2003CB515505)

摘　　要：目的研究血红素氧合酶-1(HO-1)抑制T淋巴细胞对血管内皮细胞的粘附作用。方法采用脂质体介导基因转染技术将PcDNA3-HO-1质粒转入血管内皮细胞。用间接免疫荧光技术在蛋白质水平检测HO-1在血管内皮细胞上的表达;用γ-干扰素(INF-γ)活化血管内皮细胞;用CFSE标记植物血凝素(PHA)活化的JurkatT细胞;采用粘附阻断实验观察转染HO-1的血管内皮细胞和JurkatT细胞间的粘附作用。结果HO-1可在人血管内皮细胞系中稳定表达;转染HO-1的活化内皮细胞与JurkatT细胞的粘附作用显著下降,CFSE标记的阳性率为21.24%,而未转染的对照组CFSE标记的阳性率为56.16%,两组相比,差异有统计学意义(P<0.05);应用HO-1抑制剂ZnPP后,粘附抑制作用消失。结论HO-1可以明显抑制T淋巴细胞对血管内皮细胞的粘附作用。Objective To investigate the expression of HO-1 on the endothelial cells and its protective role in adhesion between endothelial cells and Jurkat T cells. Methods The recombinant plasmid pcDNA3-HO-1 was transfected into endothelial cells, and pcDNA3-HO-1 was expressed in the endothelial cells under G418 selection. Indirect fluorescent staining was employed to examine the expression of HO-1 protein. Then endothelial cells primed by interferon-γ were cultured with Jurkat T cells labeled by CFSE. The number of adhesive Jurkat T cells was determined by FCM to evaluate the adhesion effect. Results The expression of HO-1 on endothelial cells conferred a significant protection against Jurkat T cells medicated adhesions, and the rate of Jurkat T cells adhesions was reduced to 21.24% in contrast to 56.16% in the control group ( P < 0.05 ). After using ZnPP, the inhibitor HO-1, the rate of Jurkat T cells adhesions was recovered to 48.90% . The binding activities between endothelial cells and Jurkat T cells could be blocked by the HO-1 expression. Conclusions HO-1 can directly protect endothelial cells primed by interferon-γ from Jurkat T cells adhesions. These results indicate that transgenic expression of HO-1 may be useful to prevent lymphocytes from responding to endothelial cells.

关 键 词：T淋巴细胞 粘附作用 血红素氧合酶-1 Jurkat 间接免疫荧光技术 血管内皮细胞系 HO-1 基因转染技术 CFSE 粘附抑制作用 脂质体介导 INF-γ γ-干扰素 植物血凝素 水平检测 实验观察 稳定表达 ZNPP T细胞 阳性率 蛋白质 活化 

分 类 号：R617[医药卫生—外科学]