风湿性疾病患者滑膜培养上清液中IL-1、TNF-α及PGE_2的动态观察  

IL-1 and TNF-α Bioassay and PGE_2 Immunoassay of Supernatant of Cultured Synovia in Patients with Rheumatic Diseases

作　　者：于孟学[1] 张丽华[1] 史立[1] 甘晓丹[1] 李东兵 董怡[1] 田淑芳[2] 王秀平[2] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院,北京100730 [2]中国预防医学科学院病毒学研究所

出　　处：《中国医学科学院学报》1994年第3期192-196,共5页Acta Academiae Medicinae Sinicae

摘　　要：通过关节镜技术采取膝关节滑膜并进行培养，分别于第1、4、7、14、28天收获上清液，测定33例上清液IL－1生物活性，其中类风湿关节炎（RA）11例：测定3O例TNF－α生物活性，RAg例；测定44例PGE2含量，RA11例。结果表明：IL－1增殖指数（GI）、TNF－α杀伤率（KR），RA组明显高于非滑膜炎组（对照组），两组比较差异显著（P＜0．05）；且IL－1与TNF－α高值出现的滑膜培养时间有很好的一致性。RA组使用改变病情药（DMARDs），特别是联合治疗的患者，IL－1及TNF－α活性有被压低的趋势。PGE2含量，RA＋滑膜炎组明显高于骨性关节炎＋非滑膜炎组，两组比较差异显著（P＜0．01）。提示细胞因子IL－1及TNF－α在RA发病机理中占有重要地位。本实验结果或许为类风湿新的治疗途径和战略提供一些线索。Using arthroscopy technique, synovia were adopted and cultured, and the supernatantwas harvested on 1, 4, 7, 14 and 28 days. The bioactivity of supernatant IL-1 were measured in 33 cases (RA 11 cases), TNF-α in 30 cases (RA 9 cases); the content of supernatant PGE, were measured in 44 cases (RA 11 cases ). The results showed that IL-1Growth Index (GI) and TNF a Killing Rate (KR) in RA group are higher than in non-syn-ovitis group (negative control) with statistically significant difference and that the culturedtime of the IL-1 highest value is consistent with TNF-a. In RA group, the cases treated withdisease-modifying anti-rheumatic drugs (DMARDs), especially using the combined therapy,the activity of IL-1 and TNF-a decreased. The contents of PGE, in RA+ synovitis groupwere higher than osteoarthritis (OA) + nonsynovitis group with statistically significant difference. In conclusion, cytokines such as IL-1 and TNF-α have very important status in thepathogenesis of RA. This work provides some clues for new approaches and strategies in thetreatment of RA.

关 键 词：关节炎 类风湿性 白细胞介素1 

分 类 号：R593.220.2[医药卫生—内科学]