扩增大片段DNA的PCR方法  

An Approach for PCR Amplification of Long DNA Fragments

作　　者：张敏磊[1] 左瑾[1] 徐晓石 方福德[1] 陈兰英[1] 

机构地区：[1]中国医学科学院,中国协和医科大学基础医学研究所,中国医学科学院心血管病研究所

出　　处：《中国医学科学院学报》1994年第5期348-351,共4页Acta Academiae Medicinae Sinicae

基　　金：国家"八五"攻关项目;教委博士点基金

摘　　要：本实验通过比较两种缓冲体系对ＰＣＲ扩增产物的影响，提出一种适合扩增大片段ＤＮＡ使用的缓冲体系，它与常规使用的缓冲体系的主要区别在于前者不含ＫＣｌ，使用这种缓冲体系扩增的大片段ＤＮＡ特异性强，且重复性好。The polymerase chain reaction(PCR)technique, evolved as a standard laboratory technique, has a major limitation: its inability to efficiently amplify fragments higher than 1.5kb.In this paper, PCR conditions allowing the efficient amplification of long DNA fragments, especially a new proper buffer system, have been developed. The difference between the new PCR reaction buffer system and standard reaction system is that no KCl is included in the former. The results indicated that specificity and reproductility for amplifying long DNA fragments by using new buffer system are satisfactory.

关 键 词：聚合酶链反应 DNA 缓冲体系 

分 类 号：R446.1[医药卫生—诊断学]