SARS患者尸检肺组织的TGF-β检测  

Detection of TGF-β in the Autopsy Lung Tissue from SARS Patients

作　　者：周经姣[1] 林汉良[2] 方丹云[1] 吴惠茜[2] 晏辉钧[1] 赵卫[3] 龙北国[3] 江丽芳[1] 

机构地区：[1]中山大学中山医学院微生物学教研室 [2]中山大学中山医学院病理学教研室,广东广州510080 [3]南方医科大学微生物学教研室,广东广州510515

出　　处：《中国病毒学》2005年第3期225-227,F003,共4页Virologica Sinica

基　　金：国家自然科学基金资助项目(30340013);广东省自然科学基金资助项目(532014202028)

摘　　要：为了研究TGFβ1在严重急性呼吸综合征(Severeacuterespiratorysyndrome,SARS)尸检肺组织中的表达情况及其在患者肺组织损伤中的可能作用,对2例SARS尸检肺组织进行病理学观察;应用免疫组化方法检测TGFβ1在尸检肺组织及对照肺组织中的表达情况,并进行半定量分析。结果显示病例一尸检肺组织主要病理改变为弥漫性肺泡损伤,透明膜形成及渗出性炎症。病例二尸检肺组织除了上述改变外,还伴有肺泡间质纤维增生和肺泡早期纤维化等机化性肺炎改变。TGFβ1平均灰度值在SARS患者肺组织为103.43±0.62;小叶性肺炎组织为131.47±2.64;正常肺组织中为144.24±0.09。3组比较有显著差别(P值<0.05)。SARS病毒感染后可引起急性肺间质和肺泡渗出性炎症,中后期病例还伴有肺泡间质纤维增生和肺泡早期纤维化;SARS患者肺组织损伤及纤维化与SARS冠状病毒感染后TGFβ1表达增强有关,提示抗TGFβ1治疗在SARS患者肺损伤、纤维化的预防、治疗过程中可能具有一定的临床意义。The expression of TGF and its role in the autopsy lung tissue of patients with Severe acute respiratory syndrome (SARS)was investigated .Pathological features of 2 autopsy lung tissues of SARS cases were studied by light microscope. TGF-β-1 expression in autopsy lung tissues from patients of SARS and control lung tissues was analyzed by semiquantitative method using immunohistochemistry staining. In one case, the major pathological changes of autopsy lung tissue were diffuse alveolar damage ,hyaline membrane formed and alveolar exudative inflammation. In the other case, early pulmonary fibrosis and alveolar organization were presented except degeneration and exudation. The mean greym of TGF-β-1 in autopsy lung tissues of SARS was 103.43±0.62, 131.47±2.64 in lung tissue of lobular pneumonia, 144.24±0.09 in normal lung tissue. There were significantly differences among three groups(P<0.05). The SARS-coronavirus can lead to acute pulmonary interstitial and alveolar exudative inflammation. In addition, the alveolar cell proliferation and early pulmonary fibrosis were presented during the middle and later stages of SARS. The pulmonary lesion and fibrosis in the patients of SARS appear to be associated with the overexpression of TGF-β-1. It means that anti-TGF-β-1 therapy may have a certain clinical significance on the prevention and treatment of pulmonary fibrosis of SARS.

关 键 词：严重急性呼吸综合征 肺损伤 纤维化 TGF-Β1 

分 类 号：R511.9[医药卫生—内科学]